文献类型： 外文期刊
作者： Han, Xiaoxia 1 ; Min, Ziyang 1 ; Wei, Minghua 2 ; Li, Yongqi 1 ; Wang, Duanhua 1 ; Zhang, Zhuqing 1 ; Hu, Xinjun 1 ; Kong, Qiusheng 2 ;
作者机构： 1.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha, Peoples R China
2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan, Peoples R China
关键词： Cucurbita moschata; Fruit traits; Genotyping by sequencing; High-density genetic map; QTLs
期刊名称：EUPHYTICA （ 影响因子：2.185； 五年影响因子：2.387 ）
ISSN： 0014-2336
年卷期： 2022 年 218 卷 8 期
页码：
收录情况： SCI
摘要： Pumpkin is a popular vegetable crop and exhibits a broad diversity in fruit shape and size. Fruit-related traits are the decisive factors determining consumer acceptance and market value of pumpkin cultivar. As a result, deciphering the genetic basis of fruit-related traits is of great importance for pumpkin breeding. To address this problem, a F2 population was generated by two Cucurbita moschata inbred lines with contrasting fruit shapes, and genotyping by sequencing approach was used to construct a genetic map and localize the QTLs underlying the fruit-related traits in this study. The results showed that a high-quality genetic map was constructed for pumpkin, which comprised of 2413 bins and spanned a total length of 2252.10 cM with an average genetic distance of 0.94 cM. Thirty significant QTLs with moderate or small effects were identified for seven fruit-related traits, including fruit length, fruit diameter, fruit shape index, fruit weight, fruit flesh thickness, seed cavity size, and total soluble solids content. Co-locations were observed between the QTLs underlying different traits, demonstrating that pleiotropic effect plays an important role in genetic control of fruit-related traits. The identified QTLs provide valuable information for further fine mapping of the related genes and pumpkin breeding programs with the aim of improving fruit quality.
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