Optimization and application of induction technology of double haploid in pumpkin (Cucurbita moschata D.)
文献类型： 外文期刊
作者： Min, Ziyang 1 ; Wu, Qi 2 ; Li, Jiajia 5 ; Chen, Shuitian 1 ; Li, Yongqi 1 ; Han, Xiaoxia 1 ; Zou, Tian 4 ; Hu, Xinjun 1 ;
作者机构： 1.Hunan Acad Agr Sci, Dept Vegetable Res Inst, Changsha 410125, Peoples R China
2.Shaoyang Polytech, Dept Bioengn, Shaoyang 422000, Peoples R China
3.Vegetable Breeding Ctr, Yuelushan Lab, Changsha 410128, Peoples R China
4.Hunan Biol & Electromech Polytech, Changsha 410125, Peoples R China
5.Hunan Xingshu Breed Ind Co Ltd, Changsha 410100, Peoples R China
关键词： Pumpkin; Macrospore; Haploid; Identification
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：1.9； 五年影响因子：2.1 ）
ISSN： 1054-5476
年卷期： 2025 年 61 卷 1 期
页码：
收录情况： SCI
摘要： Haploid breeding technology is an important method of rapid breeding of homozygous inbred lines. Only two generations are needed to obtain inbred lines with stable traits and completely homozygous genotypes. Based on previous research, the effects of different disinfection methods and different genotypes on embryoid induction were further explored; the regeneration of pumpkin embryo blastocyst plants was observed; the effects of plant size, seedling refining method, seedling refining environment, transplanting method, and post-transplanting management on the transplanting survival of regenerated plants were studied; and the ploidy of regenerated plants by flow cytometry and field identification was analyzed. Results indicated that disinfection with 3.0% sodium hypochlorite solution for 3.0 min was an ideal disinfection method to kill the placental tissue while maintaining the viability of the ovules. Significant differences in the induction rate of embryoid were found among different genotypes, and the highest induction rate was 36.1% in 264-2-1. The complete formation of regenerated plants through embryoid development was recorded. The survival rate of transplanting regenerated plants can reach more than 85% by initially half-opening the bottle and then completely opening the bottle. Flow cytometry identification and agronomic traits field observation showed that the researchers successfully obtained the pumpkin doubled haploid lines by megaspore culture technology. The technology system of pumpkin macrospore culture established in this study will promote the application of pumpkin haploid breeding technology to breeding practices.
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