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A novel single-base mutation in CaSGR1 confers the stay-green phenotype in pepper

文献类型： 外文期刊

作者： Liu, Yuhua 1 ; Ou, Lijun 3 ; Liu, Zhoubin 3 ; Lyu, Junheng 2 ; Wang, Jing 2 ; Song, Jingshuang 2 ; Yang, Bozhi 3 ; Chen, Wenchao 2 ; Yang, Sha 2 ; Liu, Wei 1 ; Zou, Xuexiao 3 ; Zhang, Zhuqing 2 ;

作者机构： 1.Hengyang Normal Univ, Coll Life Sci & Environm, Hengyang 421008, Henan, Peoples R China

2.Hunan Acad Agr Sci, Vegetable Inst, Changsha 410125, Hunan, Peoples R China

3.Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China

4.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Hunan, Peoples R China

关键词： Pepper; Stay-green trait; Chlorophyll degradation; CaSGR1; Hormone transduction-related transcription factors

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.4 ）

ISSN： 2095-9885

年卷期： 2023 年 9 卷 2 期

页码：

收录情况： SCI

摘要： The stay-green trait is of considerable importance in extending the shelf life of green pepper fruit (Capsicum annuum L.) and in enhancing the appearance of ornamental plants. The study revealed the genetic and regulatory mechanisms of the stay-green trait in pepper, which will aid in the selection of ornamental pepper varieties. In this study, a pepper mutant with stay-green fruit named TNX348 was identified from a germplasm resource bank. Two segregating populations were constructed using the stay-green mutant TNX348 and then used in bulked segre-gant analysis combined with RNA sequencing and linkage analyses. The causal gene of the stay-green trait was mapped to an approximately 131-kb region, and a senescence-induced chloroplast protein gene, CaSGR1 (Capana01g000359), was identified as a candidate gene. Sequencing analysis revealed a G -> A single-base mutation of CaSGR1 in TNX348 that led to early termination of translation. Based on the single-base mu-tation, a single nucleotide polymorphism (SNP) marker co-segregating with the stay-green trait was developed. Furthermore, in transcriptome analysis, expression patterns of 11 hormone transduction-related transcription factors, such as abscisic acid-insensitive (ABI), abscisic acid-responsive element-binding factor (ABF), and NAC transcription factor, were similar or opposite to that of CaSGR1. The results indicated that the transcription factors might mediate chlorophyll degradation by regulating the expression of CaSGR1.

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