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A Sensitive Method for Detecting Beauveria bassiana, an Insecticidal Biocontrol Agent, Population Dynamics, and Stability in Different Substrates

文献类型： 外文期刊

作者： Gu, Zepei 1 ; Chen, Lijie 2 ; Zhang, Weixing 2 ; Su, Pin 1 ; Zhang, Deyong 1 ; Du, Xiaohua 1 ; Peng, Qianze 2 ; Liu, Zhuoxin 2 ; Liao, Xiaolan 1 ; Liu, Yong 2 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

2.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Peoples R China

3.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China

期刊名称：CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.9 ）

ISSN： 1712-9532

年卷期： 2023 年 2023 卷

页码：

收录情况： SCI

摘要： Beauveria bassiana is a well-known insecticidal biocontrol agent. Despite its broad field applications, its survival, colonization, and stability under field conditions remained unclear, mainly due to the lack of a quick and reliable detection method. In this study, we developed a quantitative real-time PCR technology to monitor the stability and population dynamics of B. bassiana in different substrates (water, soil, and on the cotton leaves surface), different spores of B. bassiana applied on Chinese cabbage leaves surface, and the lethality of Pieris rapae spraying with different spores of B. bassiana. Our results showed a decreased concentration of B. bassiana DNA in all three substrates from the 1st day till 9th day of post inoculation (dpi) period, possibly due to the death of B. bassiana. After this decrease, a quick and significant rebound of B. bassiana DNA concentration was observed, starting from the 11th dpi in all three substrates. The B. bassiana DNA concentration reached the plateau at about 13th dpi in water and 17th dpi in the soil. On cotton leaves surface, the B. bassiana DNA concentration reached the highest level at the 17th dpi followed by a small decline and then stabilized. This increase of DNA concentration suggested recovery of B. bassiana growth in all three substrates. We found that the most suitable killing effectiveness of P. rapae was the 1.0 x 10(7) spores/mL of B. bassiana. In summary, we have established a detection technology that allows a fast and reliable monitoring for the concentration and stability of B. bassiana under different conditions. This technology can benefit and help us in the development of proper management strategies for the application of this biocontrol agent in the field.

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