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Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED

文献类型： 外文期刊

作者： Lu, Ding-Yi-Hui 1 ; Liao, Jin-Yu 2 ; Fajar, Anugerah 3 ; Chen, Jian-Bin 2 ; Wei, Yan 2 ; Zhang, Zhan-Hong 5 ; Zhang, Zhuo 2 ; Zheng, Li-Min 2 ; Tan, Xin-Qiu 2 ; Zhou, Xu-Guo 3 ; Shi, Xiao-Bin 1 ; Liu, Yong 1 ; Zhang, De-Yong 1 ;

作者机构： 1.Hunan Univ, Coll Biol, Subcoll Longping, Grad Sch, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Peoples R China

3.Univ Kentucky, Dept Entomol, Lexington, KY USA

4.Indonesia Inst Sci, Res Ctr Biomat, Cibinong, Indonesia

5.Hunan Acad Agr Sci, Inst Vegetable, Changsha, Peoples R China

关键词： Bemisia tabaci; tomato chlorosis virus; tomato yellow leaf curl virus; single infection; co-infection

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Tomato disease is an important disease affecting agricultural production, and the combined infection of tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) has gradually expanded in recent years, but no effective control method has been developed to date. Both viruses are transmitted by Bemisia tabaci Mediteranean (MED). Previously, we found that after B. tabaci MED was fed on ToCV-and TYLCV-infected plants, the transmission efficiency of ToCV was significantly higher than that on plants infected only with ToCV. Therefore, we hypothesize that co-infection could enhance the transmission rates of the virus. In this study, transcriptome sequencing was performed to compare the changes of related transcription factors in B. tabaci MED co-infected with ToCV and TYLCV and infected only with ToCV. Hence, transmission experiments were carried out using B. tabaci MED to clarify the role of cathepsin in virus transmission. The gene expression level and enzyme activity of cathepsin B (Cath B) in B. tabaci MED co-infected with ToCV and TYLCV increased compared with those under ToCV infection alone. After the decrease in cathepsin activity in B. tabaci MED or cathepsin B was silenced, its ability to acquire and transmit ToCV was significantly reduced. We verified the hypothesis that the relative expression of cathepsin B was reduced, which helped reduce ToCV transmission by B. tabaci MED. Therefore, it was speculated that cathepsin has profound research significance in the control of B. tabaci MED and the spread of viral diseases.

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