您好，欢迎访问湖南省农业科学院 机构知识库！

CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

文献类型： 外文期刊

作者： Lu, Qinghua 1 ; Luo, Xiangwen 1 ; Yang, Xiao 1 ; Zhou, Tong 3 ; Zhang, Yu 2 ; Lan, Ying 3 ; Zhang, Deyong 1 ; Zheng, Limin 2 ; Li, Yixin 1 ; Li, Li 1 ; Zhang, Songbai 1 ; Liu, Yong 1 ;

作者机构： 1.Hunan Univ, Longping Branch Biol Coll, Changsha, Peoples R China

2.Hunan Acad Agr Sci, State Key Lab Hybird Rice, Changsha, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety, Nanjing, Peoples R China

关键词： CRISP; Cas9; vacuolar ATPase subunit d; phytohormone biosynthesis; virus resistance; rice

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of OsV-ATPase d in rice were generated using the CRISPR/Cas9 system, and mutation of OsV-ATPase d did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that OsV-ATPase d is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of OsV-ATPase d significantly increased JA and ABA biosynthesis and resistance against Southern rice black-streaked dwarf virus (SRBSDV), but it decreased resistance against Rice stripe virus (RSV) in rice. The data presented in this study reveal that OsV-ATPase d mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台