Problems Encountered with the Selection of Cucumber Mosaic Virus (CMV) Isolates for Resistance Breeding Programs
文献类型： 外文期刊
作者： Zhang, Deyong 2 ; Tan, Xinqiu 2 ; Willingmann, Peter 1 ; Adam, Guenter 1 ; Heinze, Cornelia 1 ;
作者机构： 1.Univ Hamburg, Biocentre Klein Flottbek & Bot Garden, D-22609 Hamburg, Germany
2.Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China
关键词： Cucumber mosaic virus;pseudorecombinants;resistance breaking
期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）
ISSN： 0931-1785
年卷期： 2011 年 159 卷 9 期
页码：
收录情况： SCI
摘要： Among the Chili breeding lines from the Asian Vegetable Research Center, two were chosen for the screening of a larger selection of Cucumber mosaic virus (CMV) isolates, mainly from Asian countries. The chili line (VC246) showed a resistance against several CMV-isolates and was compared with chili line VC27a that was susceptible to CMV infection. Among the 28 CMV isolates, five were identified as resistance breaking (AN-like) and non-resistance breaking (P3613-like) for the line VC246, whereas all isolates could establish a systemic infection on VC27a. However, further testing revealed that resistance in VC246 was also dependent on the way of inoculation and the inoculums itself. Graft inoculation could overcome the resistance, and the inoculation with isolated viral RNA resulted in no infection at all on the resistant chili line, independent of the virus isolate. Using a pseudo-recombinant approach, we identified RNA2 of resistance breaking isolates as responsible for systemic infection and confined the area within RNA2 to the 3' terminal part including the ORF 2b. Sequence alignments of that area revealed eight distinct mutations on amino acid level, which was present either in resistance or non-resistance breaking isolates. A reversion from the P3613-like to the AN-like sequence of two of these mutations induced no effect on Capsicum sp., but induced symptoms on several tobacco species distinct from those induced by the wild-type virus. However, pseudorecombinants, each generated from sets of two different AN-like isolates, which were expected to infect VC246 systemically, did not indicating that probably RNA2 must be in a specific context to have the effect. In this case, a generalized attribution of functions to single amino acid exchanges might be impossible or at least extremely difficult.
- 相关文献
作者其他论文 更多>>
-
Host metabolites explain microbiome variation between different rice genotypes
作者：Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Kang, Houxiang;Peng, Qianze;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Zhang, Chi;Lei, Ziling;Liu, Yong;Zhang, Deyong;Tang, Qianjun;Cernava, Tomislav
关键词：Plant genotype; Plant microbiome; Genetic control; Plant metabolites; Phenylpropanoids
-
Analysis of the Antagonistic Effect of Dibutyl Phthalate on Magnaporthe oryzae through Bioassay and Omics Data
作者：Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue
关键词：dibutyl phthalate; antifungal activity; Magnaportheoryzae; transcriptome; metabolome
-
Transcriptome Analysis Reveals the Role of OsCBM1 in Rice Defense Against Xanthomonas oryzae pv.oryzae
作者：Bie, Shuaijun;Liu, Yong;Zhang, Deyong;Xiao, Youlun;Liu, Yong;Peng, Jing;Gao, Yang;Li, Xiaojuan;Tan, Xinqiu;Huang, Renyan;Zhang, Deyong;Zhang, Li;He, Xiaomin;Xie, Hongjun;Huang, Renyan;Zhang, Deyong
关键词：malectin-like domain-containing protein;
OsCBM1 ;Xanthomonas oryzae pv. oryzae ; reactive oxygen species; phytohormones; rice -
A viral movement protein mediates host volatile biosynthesis to co-attract vectors and non-vectors and enhances viral infection
作者：Li, Shijun;Luo, Xiangwen;Zhang, Zhanhong;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Zhou, Xuguo;Wang, Xin;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Qu, Xiaoxin
关键词：pepper veinal yellows virus; vector; non-vector; volatiles; tetra-interaction
-
Microbiome Migration from Soil to Leaves in Maize and Rice
作者：Ma, Jiejia;Chen, Silu;Liu, Zhuoxin;Zhang, Chi;Lei, Ziling;Su, Pin;Zhang, Deyong;Liu, Yong;Ma, Jiejia;Peng, Qianze;Chen, Silu;Liu, Zhuoxin;Zhang, Weixing;Zhang, Chi;Du, Xiaohua;Sun, Shue;Peng, Weiye;Lei, Ziling;Zhang, Limei;Su, Pin;Zhang, Deyong;Liu, Yong;Ma, Jiejia;Peng, Qianze;Chen, Silu;Liu, Zhuoxin;Zhang, Weixing;Zhang, Chi;Du, Xiaohua;Sun, Shue;Peng, Weiye;Lei, Ziling;Zhang, Limei;Su, Pin;Zhang, Deyong;Liu, Yong
关键词：plant microbiome; soil microbiome; synthetic microbial community; rice; maize; Burkholderiales
-
The effect of developmental stages on microbiome assembly in the phyllosphere and rhizosphere of rice grown in urban area soil
作者：Peng, Qianze;Zhang, Deyong;Peng, Qianze;Sun, Shu'e;Du, Xiaohua;Zhu, Feiying;Peng, Weiye;Liu, Yong;Su, Pin;Zhang, Deyong;Peng, Qianze;Sun, Shu'e;Du, Xiaohua;Zhu, Feiying;Peng, Weiye;Liu, Yong;Su, Pin;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Ma, Jiejia;Chen, Silu;Gao, Liming;Liu, Yong;Su, Pin;Zhang, Deyong;Liu, Xian;Cernava, Tomislav
关键词：Phyllosphere; Rhizosphere; Urbanization; Rice; Microbiome assembly; Plant Microbiome
-
Molecular Insights into the Role of the MET30 Protein and Its WD40 Domain in Colletotrichum gloeosporioides Growth and Virulence
作者：Wu, Fei;Huang, Longhui;Liu, Sizhen;Chen, Yue;Guo, Sheng;Tan, Xinqiu;Wu, Fei;Sun, Qianlong;Huang, Longhui;Liu, Sizhen;Chen, Yue;Zhang, Xin;Li, Chenggang;Guo, Sheng;Tan, Xinqiu;Sun, Qianlong;Chen, Yue;Zhang, Xin;Li, Chenggang;Tan, Xinqiu
关键词：
Phytopathogenic fungi ; MET30 protein; WD40 domain; pathogenicity; stress response
