文献类型： 外文期刊

作者： Su, Pin ¹ ; Kang, Houxiang ³ ; Peng, Qianze ⁴ ; Peng, Weiye ¹ ; Sun, Shu'e ¹ ; Du, Xiaohua ¹ ; Zhang, Chi ⁶ ; Lei, Ziling ⁶ ; Bai, Lianyang ¹ ; Tang, Qianjun ⁷ ; Liu, Yong ¹ ; Cernava, Tomislav ⁸ ; Zhang, Deyong ¹ ;

作者机构： 1.Hunan Acad Agr Sci, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China

3.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

4.Yuelushan Lab, Changsha 410016, Peoples R China

5.Hainan Univ, Sch Trop Agr & Forestry, Haikou 570228, Peoples R China

6.Hunan Univ, Coll Biol, Longping Branch, Changsha 410082, Peoples R China

7.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

8.Univ Southampton, Fac Environm & Life Sci, Sch Biol Sci, Southampton SO17 1BJ, England

关键词： Plant genotype; Plant microbiome; Genetic control; Plant metabolites; Phenylpropanoids

期刊名称：MICROBIOME （ 影响因子：12.7； 五年影响因子：16.6 ）

ISSN： 2049-2618

年卷期： 2025 年 13 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPlants live in close association with microbial communities that support their health and growth. Previous research has indicated that the composition of these communities can differ between genotypes of the same plant species. Host-related factors causing this variation are mostly unknown. Microbiome genes, or M genes in short, are host genes that are involved in shaping the microbiome. We hypothesized that specific M genes are responsible for microbiome variation between rice genotypes and that it is connected to plant metabolites controlled by these genes.ResultsOur study was aimed at identifying plant metabolites driving genotype-specific microbiome assembly and establishing a link to host genetics. Targeted metabolite quantification was combined with microbiome profiling of the rice phyllosphere microbiome, association analyses on single-nucleotide polymorphism (SNP) level, and genetic modifications to validate microbiome-shaping effects of the discovered M genes. Targeted metabolite quantifications revealed that phenylpropanoid concentrations in rice leaves can substantially differ among 110 representative genotypes grown under the same, controlled conditions. Redundancy analyses (RDA) showed that these metabolites can explain 35.6% of the variance in their microbiomes. Further verification experiments resulted in the identification of two M genes. OsC4H2 and OsPAL06 are both plant genes with microbiome-shaping effects, mainly via their role in ferulic acid biosynthesis. Targeted gene mutation experiments confirm that distinct phyllosphere-associated bacterial groups are highly responsive to the discovered M genes.ConclusionThis study provides detailed insights into the links between host genetics and microbiome variation in plants. Knowledge about host genes that are in control of the microbiome paves the way for microbiome engineering and targeted plant breeding approaches.CSbjuAvs7sungUB_-bFGJyVideo AbstractConclusionThis study provides detailed insights into the links between host genetics and microbiome variation in plants. Knowledge about host genes that are in control of the microbiome paves the way for microbiome engineering and targeted plant breeding approaches.CSbjuAvs7sungUB_-bFGJyVideo Abstract