The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin
文献类型： 外文期刊
作者： Wu, Jing 1 ; Yang, Chenglin 1 ; Yang, Mengran 1 ; Liang, Zengenni 3 ; Wu, You 1 ; Kong, Xiangyi 1 ; Fan, Hui 1 ; Wang, Siqi 1 ; Ning, Can 1 ; Xiao, Wenguang 1 ; Jin, Ye 4 ; Yi, Jine 1 ; Yuan, Zhihang 1 ;
作者机构： 1.Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Livestock & Poultry Hlth Care, Changsha 410128, Peoples R China
2.Hunan Collaborat Innovat Ctr Anim Prod Safety, Changsha 410128, Peoples R China
3.Dept Hunan Agr Prod Proc Inst, Changsha 410128, Peoples R China
4.Hunan Pujian Biol Technol Co Ltd, Changsha 410128, Peoples R China
关键词： Citrinin; ROS; ER stress; AMPK; Cell cycle arrest; Apoptosis
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）
ISSN： 0147-6513
年卷期： 2022 年 237 卷
页码：
收录情况： SCI
摘要： Citrinin, a secondary metabolite, can pose serious risks to the environment and organisms, but its hepatotoxic mechanisms are still unclear. Histopathological and ultrastructural results showed that citrinin-induced liver injury in Kunming mice, and the mechanism of citrinin-induced hepatotoxicity was studied in L02 cells. Firstly, citrinin mades L02 cell cycle arrest in G2/M phase by inhibition of cyclin B1, cyclin D1, cyclin-dependent kinases 2 (CDK2), and CDK4 expression. Secondly, citrinin inhibits proliferation and promotes apoptosis of L02 cells via disruption of mitochondria membrane potential, increase Bax/Bcl-2 ration, activation of caspase-3, 9, and enhance lactate dehydrogenase (LDH) release. Then, citrinin inhibits superoxide dismutase (SOD) activity and increases the accumulation of malondialdehyde (MDA) and reactive oxygen species (ROS), resulting oxidative damage in L02 cells; upregulates the protein expression of binding immunoglobulin protein (Bip), C/EBP homologous protein (CHOP), PKR-like ER kinase (PERK) and activating transcription factor6 (ATF6), inducing ER stress in L02 cells; increases the phosphorylation of AMP-activated protein kinase (AMPK) and decreases the content of adenosine-triphosphate (ATP), activating AMPK pathway in L02 cells. Eventually, pretreatment with NAC, an ROS inhibitor, alleviates citrinin-induced cell cycle G2/M arrest and apoptosis by inhibiting ROSmediated ER stress; pretreatment with 4-PBA, an ER stress inhibitor, reversed ER stress and p-AMPK; pretreatment with dorsomorphin, an AMPK inhibitor, decreases citrinin-induced cell cycle G2/M arrest and apoptosis. In summary, citrinin induces cell cycle arrest and apoptosis to aggravate liver injury by activating ROS-ER stressAMPK signaling pathway.
- 相关文献
作者其他论文 更多>>
-
Melatonin Alleviates T-2 Toxin-Induced Intestinal Injury by Enhancing Gut Barrier Function and Modulating Microbiota in Weaned Piglets
作者：Wu, You;Xiao, Wenguang;Xiao, Bo;Wang, Yongkang;Li, Yuanyuan;Wu, Aoao;Zhang, Qike;Liu, Xiaofang;Liu, Shuiping;Yuan, Zhihang;Yi, Jine;Wu, Jing;Liang, Zengenni
关键词：melatonin; T-2 toxin; gut barrier function; gut microbiota; SCFAs
-
Koumine mediates the generation of mtROS through the IP3R1-GRP75-VDAC1 complex to improve Citrinin induced intestinal inflammation
作者：Li, Yuanyuan;Liu, Xiaofang;Zhang, Qike;Jiang, Zonghan;Yang, Chenglin;Ni, Jie;Deng, Siqi;Yi, Jine;Wu, Jing;Sun, Zhiliang;Yuan, Zhihang;Zhang, Weiqing;Liang, Zengenni;Liang, Zengenni
关键词：Koumine; Citrinin; Inflammatory bowel disease; Pyroptosis
-
Citrinin-Induced Intestinal Onset of Pyroptosis via the IP3R1-GRP75-VDAC1 Complex-Mediated Mitochondrial Oxidative Stress
作者：Li, Yuanyuan;Zhang, Qike;Liu, Xiaofang;Wang, Yongkang;Yang, Chenglin;Wu, You;Xiao, Bo;Feng, Yiya;Wu, Aoao;Yi, Jine;Wu, Jing;Yuan, Zhihang;Wu, Jing;Yuan, Zhihang;Liang, Zengenni;Liang, Zengenni
关键词：citrinin; MAM; pyroptosis; mitochondrial oxidative stress; intestinal
-
Melatonin alleviates T-2 toxin-induced oxidative damage, inflammatory response, and apoptosis in piglet spleen and thymus
作者：Ning, Can;Xiao, Wenguang;Wu, You;Fan, Hui;Wang, Siqi;Kong, Xiangyi;Wang, Yongkang;Wu, Aoao;Li, Yuanyuan;Yuan, Zhihang;Wu, Jing;Yang, Chenglin;Liang, Zengenni;Liang, Zengenni
关键词：T-2 toxin; Melatonin; Spleen; Thymus; Immunotoxicity
-
Apoptosis mediated by crosstalk between mitochondria and endoplasmic reticulum: A possible cause of citrinin disruption of the intestinal barrier
作者：Li, Yuanyuan;Wang, Yongkang;Jiang, Zonghan;Yang, Chenglin;Wu, You;Wu, Aoao;Zhang, Qike;Liu, Xiaofang;Xiao, Bo;Feng, Yiya;Wu, Jing;Yuan, Zhihang;Liang, Zengenni;Wu, Jing;Yuan, Zhihang;Liang, Zengenni
关键词：Citrinin; MAM; Intestinal; Intestinal barrier; Intestinal microbiota; Apoptpsis
-
Koumine inhibits IL-1β-induced chondrocyte inflammation and ameliorates extracellular matrix degradation in osteoarthritic cartilage through activation of PINK1/Parkin-mediated mitochondrial autophagy
作者：Kong, Xiangyi;Ning, Can;Yang, Chenglin;Wu, You;Li, Yuanyuan;Wu, Aoao;Wang, Yongkang;Wang, Siqi;Fan, Hui;Xiao, Wenguang;Wu, Jing;Yuan, Zhihang;Liang, Zengenni;Sun, Zhiliang
关键词：Koumine; Osteoarthritis; Extracellular matrix; Mitophagy; PINK1/Parkin
-
Viola yedoensis Makino alleviates heat stress-induced inflammation, oxidative stress, and cell apoptosis in the spleen and thymus of broilers
作者：Mao, Yan;Kong, Xiangyi;Yang, Chenglin;Wang, Siqi;Fan, Hui;Ning, Can;Xiao, Wenguang;Wu, You;Wu, Jing;Yuan, Zhihang;Liang, Zengenni;Yuan, Liyun
关键词：Viola yedoensis Makino; Heat stress; Broiler; Apoptosis; Oxidative stress; Immune organs
