Viola yedoensis Makino alleviates heat stress-induced inflammation, oxidative stress, and cell apoptosis in the spleen and thymus of broilers
文献类型: 外文期刊
作者: Mao, Yan 1 ; Kong, Xiangyi 1 ; Liang, Zengenni 2 ; Yang, Chenglin 1 ; Wang, Siqi 1 ; Fan, Hui 1 ; Ning, Can 1 ; Xiao, Wenguang 1 ; Wu, You 1 ; Wu, Jing 1 ; Yuan, Liyun 3 ; Yuan, Zhihang 1 ;
作者机构: 1.Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Livestock & Poultry Hlth Care, Changsha 410128, Peoples R China
2.Hunan Acad Agr Sci, Dept Hunan Agr Prod Proc Inst, Changsha 410128, Peoples R China
3.Xiangyang Vocat & Tech Coll, Xiangyang 441050, Peoples R China
关键词: Viola yedoensis Makino; Heat stress; Broiler; Apoptosis; Oxidative stress; Immune organs
期刊名称:JOURNAL OF ETHNOPHARMACOLOGY ( 影响因子:5.4; 五年影响因子:5.3 )
ISSN: 0378-8741
年卷期: 2024 年 319 卷
页码:
收录情况: SCI
摘要: Ethnopharmacological relevance: Viola yedoensis Makino (VYM) is a traditional Chinese herbal medicine widely distributed in China. It has many pharmacological effects such as anti-inflammatory, immune regulation and anti-oxidation. However, the protective effect of VYM on the spleen and thymus of broilers induced by heat stress has rarely been reported. Aim of the study: We established a heat stress model of broilers to explore the protective effect of VYM on spleen and thymus of broilers. Materials and methods: In this experiment, a heat stress model was made by adjusting the feeding temperature of broilers. The protective effect of VYM on the spleen and thymus of heat-stressed broilers were evaluated by detecting immune organ coefficient, histological observation, Enzyme-Linked Immunosorbent Assay, production of antioxidant enzymes and peroxides, TUNEL Staining, Quantitative Real-time PCR. Results: In this study, 60 healthy male AA broilers were divided into 6 groups: Control, 4.5% VYM, HS, HS + 0.5% VYM, HS + 1.5% VYM, HS + 4.5% VYM. After 42 days of feeding, serum, spleen and thymus were collected for detection and analysis. The study revealed that heat stress can lead to pathological damage in the spleen and thymus of broilers, reduce the content of immunoglobulin and newcastle disease (ND), infectious bursal disease (IBD) antibody levels, increase the expression of inflammatory factors IL-1 beta, INF-gamma, heat shock 70 kDa protein (HSP70), heat shock 90 kDa protein (HSP90). Heat stress inhibits the activity of antioxidant enzymes CAT and SOD, promotes the production of MDA, and then lead to oxidative damage of the spleen and thymus. In addition, apoptotic cells and the ratio of Bax/Bcl-2 was increased. However, the addition of VYM to the feed can alleviate the adverse effects of heat stress on the spleen and thymus of broilers. Conclusions: This study showed that the addition of VYM to the diet could inhibit oxidative stress and apoptosis, and reduce the inflammatory damage of heat stress on the spleen and thymus of broilers. This study provides a basis for further exploring the regulatory role of VYM in heat stress-induced immune imbalance in broilers. In addition, this study also provides a theoretical basis for the development of VYM as a feed additive with immunomodulatory effects.
- 相关文献
作者其他论文 更多>>
-
PI3K/AKT/mTOR, NF-Kappa B and ERS pathway participated in the attenuation of H2O2-induced IPEC-J2 cell injury by koumine
作者:Yuan, Zhihang;Yang, Mengran;Yang, Chenglin;Kong, Xiangyi;Wu, You;Wang, Siqi;Fan, Hui;Ning, Can;Xiao, Wenguang;Sun, Zhiliang;Wu, Jing;Yuan, Zhihang;Yang, Mengran;Yang, Chenglin;Kong, Xiangyi;Wu, You;Wang, Siqi;Fan, Hui;Ning, Can;Xiao, Wenguang;Sun, Zhiliang;Wu, Jing;Sun, Zhiliang;Liang, Zengenni;Sun, Zhiliang
关键词:Koumine; IPEC-J2; Inflammation; ERS; ROS; Apoptosis; Autography; NF-?B; PI3K
-
The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin
作者:Wu, Jing;Yang, Chenglin;Yang, Mengran;Wu, You;Kong, Xiangyi;Fan, Hui;Wang, Siqi;Ning, Can;Xiao, Wenguang;Yi, Jine;Yuan, Zhihang;Wu, Jing;Yang, Chenglin;Yang, Mengran;Wu, You;Kong, Xiangyi;Fan, Hui;Wang, Siqi;Ning, Can;Xiao, Wenguang;Yi, Jine;Yuan, Zhihang;Liang, Zengenni;Jin, Ye
关键词:Citrinin; ROS; ER stress; AMPK; Cell cycle arrest; Apoptosis
-
New Mechanistic Insight into the Protective Effects of Ganoderma lucidum Polysaccharides Against Palmitic Acid-Induced Cell Damage in Porcine Intestinal Epithelial Cell Line IPEC-J2
作者:Liang, Zengenni;Guo, Jia-Jing;Shan, Yang;Yuan, Zhi-Hang;Wang, Yan;Du, Zhong-Hua;Xia, Ling-Li;Shan, Yang
关键词:Ganoderma lucidum polysaccharides; high-fat injury; STAT pathway; medicinal mushrooms; intestinal injury
-
Nobiletin, a hexamethoxyflavonoid from citrus pomace, attenuates G1 cell cycle arrest and apoptosis in hypoxia-induced human trophoblast cells of JEG-3 and BeWo via regulating the p53 signaling pathway
作者:Zhang, Mengling;Liu, Jian;Liang, Zengenni;Ding, Shenghua;Shan, Yang;Zhang, Mengling;Yu, Huanling;Zhang, Mengling;Liu, Jian;Liang, Zengenni;Ding, Shenghua;Shan, Yang;Zhang, Mengling;Liu, Jian;Liang, Zengenni;Ding, Shenghua;Shan, Yang;Zhang, Rui
关键词:Nobiletin; p53; molecular docking; molecular dynamics; spectrum; apoptosis; trophoblast cells
-
Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice
作者:Wu, Jing;Li, Jiayan;Liu, Yanwei;Liao, Xinxin;Wu, Dongyi;Chen, Yunqin;Yuan, Zhihang;Li, Rongfang;Yi, Jine;Wu, Jing;Li, Jiayan;Liu, Yanwei;Liao, Xinxin;Wu, Dongyi;Chen, Yunqin;Yuan, Zhihang;Li, Rongfang;Yi, Jine;Wen, Lixin;Wen, Lixin;Liang, Zengenni
关键词:Zearalenone; Oxidative stress; Apoptosis; Death receptor signaling pathway; Mitochondrial apoptosis signaling pathway
-
Betulinic acid attenuates cyclophosphamide-induced intestinal mucosa injury by inhibiting the NF-kappa B/MAPK signalling pathways and activating the Nrf2 signalling pathway
作者:Ou, Zhaoping;Zhu, Lijuan;Huang, Chenglong;Ma, Chaoyang;Kong, Li;Lin, Xing;Gao, Xinyu;Huang, Lin;Wen, Lixin;Yuan, Zhihang;Wu, Jing;Yi, Jine;Liang, Zengenni
关键词:Betulinic acid; Cyclophosphamide; Intestinal mucosal barrier dysfuction; NF-kappa B/MAPK pathways; Nrf2 pathway
-
Alternative Extraction Methods of Essential Oil From the Flowers of Citrus aurantium L. Var Daidai Tanaka: Evaluation of Oil Quality and Sedative-Hypnotic Activity
作者:Liang, Zengenni;Shang, Xue-bo;Li, Gao-yang;Fu, Fu-hua;Guo, Jia-jing;Shan, Yang;Su, Jin
关键词:Citrus aurantium var; daidai flowers; essential oil; hypnotic components; locomotor activity; Cl(-)influx; GABA(A)