您好，欢迎访问湖南省农业科学院 机构知识库！

A ScWIP5 gene confers fall armyworm resistance by reducing digestive enzyme activities in sugarcane

文献类型： 外文期刊

作者： Wang, Ya-ru 2 ; Yao, Yang 2 ; Chen, Yao-hui 2 ; Huang, Cheng 2 ; Guo, Yan-fang 2 ; Fang, Yong 4 ; Gao, San-ji 2 ; Hou, You-ming 1 ; Wang, Jin-da 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou 350002, Peoples R China

2.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou, Peoples R China

3.Xianghu Lab, Hangzhou, Peoples R China

4.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha, Peoples R China

关键词： plant endogenous insect-resistant gene; jasmonic acid; transgenic; protease inhibitors

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年 80 卷 4 期

页码：

收录情况： SCI

摘要： BACKGROUND: The fall armyworm, Spodoptera frugiperda, is one of the most dangerous pests to various crops. As the most crucial sugar crop, sugarcane is also constantly threatened by these pests. Plant wound-induced proteinase inhibitors (WIP) are natural defense proteins that play important roles in the defense system against insect attack. Breeding for resistance would be the best way to improve the variety characteristics and productivity of sugarcane. Screening and verification for potential plant endogenous insect-resistant genes would greatly improve the insect-resistant breeding progress of sugarcane. RESULTS: A sugarcane WIP5 gene (ScWIP5) was up-regulated 536 times after insect feeding treatment on previous published transcriptome databases. ScWIP5 was then cloned and its potential role in sugarcane resistance to fall armyworm evaluated by construction of transgenic Nicotiana benthamiana. The toxicity of ScWIP5 transgenic N. benthamiana to fall armyworm showed lower weight gain and higher mortality compared to wild-type N. benthamiana feeding group. Furthermore, the concentration of JA and NbAOC, NbAOS, and NbLOX from the Jasmin acid biosynthesis pathway was significantly induced in ScWIP5 transgenic N. benthamiana compared to the control. In addition, digestive enzyme actives from the insect gut were also evaluated, and trypsin and cathepsin were significantly lower in insects fed with ScWIP5 transgenic N. benthamiana. CONCLUSION: These results indicate that ScWIP5 might enhance insect resistance by increasing JA signal transduction processes and reducing insect digestive enzyme activities, thus impacting insect growth and development. (c) 2023 Society of Chemical Industry.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台