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The effect of chlorogenic acid, a potential botanical insecticide, on gene transcription and protein expression of carboxylesterases in the armyworm (Mythimna separata)

文献类型： 外文期刊

作者： Lin, Dong-Jiang 1 ; Zhang, Ya-Xin 1 ; Fang, Yong 2 ; Gao, San-Ji 1 ; Wang, Ran 3 ; Wang, Jin-Da 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China

4.Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China

5.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Chlorogenic acid; Proteomic; Carboxylesterase; RNAi

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 195 卷

页码：

收录情况： SCI

摘要： Chlorogenic acid (CGA) is a potential botanical insecticide metabolite that naturally occurs in various plants. Our previous studies revealed CGA is sufficient to control the armyworm Mythimna separata. In this study, we conducted a proteomic analysis of saliva collected from M. separata following exposure to CGA and found that differentially expressed proteins (DEPs) treated with CGA for 6 h and 24 h were primarily enriched in glutathione metabolism and the pentose phosphate pathway. Notably, we observed six carboxylesterase (CarE) proteins that were enriched at both time points. Additionally, these corresponding genes were expressed at levels 5.05 to 130.25 times higher in our laboratory-selected resistance strains. We also noted a significant increase in the enzyme activity of carboxylesterase following treatments with varying CGA concentrations. Finally, we confirmed that knockdown of MsCarE14, MsCarE28, and MsCCE001h decreased the susceptibility to CGA in resistance strain, indicating three CarE genes play crucial roles in CGA detoxification. This study presents the first report on the salivary proteomics of M. separata, offering valuable insights into the role of salivary proteins. Moreover, the determination of CarE mediated susceptibility change to CGA provides new targets for agricultural pest control and highlights the potential insecticide resistance mechanism for pest resistance management.

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