Biocontrol efficiency and characterization of insecticidal protein from sugarcane endophytic Serratia marcescens (SM) against oriental armyworm Mythimna separata (Walker)
文献类型： 外文期刊
作者： Lin, Dong-Jiang 1 ; Zhou, Jiang-xiong 1 ; Ali, Ahmad 1 ; Fu, Hua-ying 1 ; Gao, San-ji 1 ; Jin, Lin 2 ; Fang, Yong 3 ; Wang, Jin-da 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou 350002, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
3.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Hunan, Peoples R China
关键词： Serratia marcescens; Serralysin; Mythimna separata (Walker)
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 262 卷
页码：
收录情况： SCI
摘要： The plant endophytic bacteria are a great source of nature insecticides. However, no such endophytic bacteria have been found in sugarcane, to address this gap, we isolated and identified a strain of Serratia marcescens with moderate insecticidal activity from sugarcane. Taken armyworm Mythimna separata as example, the mortality rates of oral infection and injection infection were 47.06 % and 91 %, respectively. The SM has significant negative affect on the growth, development, and reproduction of M. separata. After determining that these insecticidal substances, 33 potential virulence proteins were screened through the identification and prediction of bacterial proteins. Later we confirmed serralysin was a vital toxic protein from SM that caused M. separata death by prokaryotic expression. In addition, we also found that the intestinal tissue cells infected with SM or serralysin were severely diseased, which may be a major factor in M. separata demise. Finally, through gene expression level, protein molecular docking, we found the aminopeptidase-N would be one of the potential receptors of serralysin. Taken together, our findings indicate that sugarcane endophyte S. marcescens may be beneficial for pest control in sugarcane and explain its insecticidal mechanism. This study provides new ideas and materials for the biological control of pests.
- 相关文献
作者其他论文 更多>>
-
Tetraniliprole resistance in field-collected populations of Tuta absoluta (Lepidoptera: Gelechiidae) from China: Baseline susceptibility, cross-resistance, inheritance, and biochemical mechanism
作者：Qu, Cheng;Yao, Jiaqi;Luo, Chen;Wang, Ran;Yao, Jiaqi;Huang, Jianlei;Che, Wunan;Fang, Yong;Fang, Yong
关键词：Tuta absoluta; Tetraniliprole; Cross -resistance; Synergism; Metabolic enzyme; Mode of inheritance
-
A ScWIP5 gene confers fall armyworm resistance by reducing digestive enzyme activities in sugarcane
作者：Hou, You-ming;Wang, Jin-da;Wang, Ya-ru;Yao, Yang;Chen, Yao-hui;Huang, Cheng;Guo, Yan-fang;Gao, San-ji;Hou, You-ming;Wang, Jin-da;Wang, Ya-ru;Fang, Yong
关键词：plant endogenous insect-resistant gene; jasmonic acid; transgenic; protease inhibitors
-
Nano-selenium promotes the product quality and plant defense of Salvia miltiorrhiza by inducing tanshinones and salvianolic acids accumulation
作者：Zhang, Yanhua;Zhang, Tong;Pan, Yanyan;Ma, Longxia;Qiang, Yi;Cao, Xiaoyan;Xu, Hongxing;Fang, Yong;Pan, Canping
关键词：Nano-Se; Danshen; Secondary metabolites; Aphids; Plant hormones
-
The effect of chlorogenic acid, a potential botanical insecticide, on gene transcription and protein expression of carboxylesterases in the armyworm (Mythimna separata)
作者：Lin, Dong-Jiang;Zhang, Ya-Xin;Gao, San-Ji;Wang, Jin-Da;Fang, Yong;Wang, Ran;Wang, Jin-Da;Wang, Ran
关键词：Chlorogenic acid; Proteomic; Carboxylesterase; RNAi
-
Detection of insecticide sensitivity and target mutation in seven populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) in China
作者：Zhu, Feiying;Luo, Zhenliang;Fang, Yong;Wang, Jingda;Cheng, Hongsong;Wang, Junhua
关键词：Spodoptera frugiperda; Insecticide sensitivity; Ace-1; Genotype; Mutation frequency
-
Nano-selenium enhances sugarcane resistance to Xanthomonas albilineans infection and improvement of juice quality
作者：Shi, Meng-Ting;Zhang, Tian-Jie;Fu, Hua-Ying;Gao, San-Ji;Wang, Jin-da;Fang, Yong;Fang, Yong;Pan, Can-Ping
关键词：Jasmonic acid; Leaf scald disease; Nano-Se; ROS; Sugarcane juice
-
Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China
作者：Wang, Ran;Zhang, Qinghe;Luo, Chen;Fang, Yong;Che, Wunan;Wang, Jinda
关键词：Bemisia tabaci; abamectin; cross-resistance; synergistic effects; metabolic enzymes; inheritance; resistance management
