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The Pentatricopeptide Repeat Protein OsPPR674 Regulates Rice Growth and Drought Sensitivity by Modulating RNA Editing of the Mitochondrial Transcript ccmC

文献类型： 外文期刊

作者： Li, Jinglei 1 ; Zhang, Longhui 2 ; Li, Chenyang 2 ; Chen, Weijun 2 ; Wang, Tiankang 2 ; Tan, Lvni 1 ; Qiu, Yingxin 1 ; Song, Shufeng 1 ; Li, Bin 2 ; Li, Li 1 ;

作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China

2.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

关键词： rice (Oryza sativa L.); RNA editing; PPR protein; drought stress adaptation; molecular breeding

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 6 期

页码：

收录情况： SCI

摘要： The P-type pentatricopeptide repeat (PPR) proteins are crucial for RNA editing and post-transcriptional regulation in plant organelles, particularly mitochondria. This study investigates the role of OsPPR674 in rice, focusing on its function in mitochondrial RNA editing. Using CRISPR/Cas9 technology, we generated ppr674 mutant and examined its phenotypic and molecular characteristics. The results indicate that ppr674 exhibits reduced plant height, decreased seed-setting rate, and poor drought tolerance. Further analysis revealed that in the ppr674 mutant, RNA editing at the 299th nucleotide position of the mitochondrial ccmC gene (C-to-U conversion) was abolished. REMSAs showed that GST-PPR674 specifically binds to RNA probes targeting this ccmC-299 site, confirming its role in this editing process. In summary, these results suggest that OsPPR674 plays a pivotal role in mitochondrial RNA editing, emphasizing the significance of PPR proteins in organelle function and plant development.

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