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Effect of wogonin, an active ingredient of Scutellariae Radix, on fish infected with Aeromonas hydrophila

文献类型： 外文期刊

作者： Cui, Jun 1 ; Zeng, Dan 1 ; Guan, Peipei 1 ; Jiang, Ning 2 ;

作者机构： 1.Hunan Normal Univ, Coll Life Sci, State Key Lab Dev Biol Freshwater Fish, Hunan Prov Key Lab Microbial Mol Biol, Changsha, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha, Hunan, Peoples R China

关键词： Wogonin; Inflammatory; Scutellariae radix; Fish; AKT2

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.2； 五年影响因子：2.5 ）

ISSN： 0967-6120

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Exposure to pathogens induces hyperactivate inflammatory immune responses, oxidative stress and even death in fish. Therefore, suppressing excessive inflammatory responses is a means of treating fish diseases. Scutellariae Radix (SR), an herbal medicine, has anti-inflammatory and antioxidant properties. However, the active ingredients of SR that produce a marked effect and their mechanisms are poorly understood. In this study, 32 active ingredients of SR and their 124 target genes associated with anti-inflammatory effect were screened. These target genes in zebrafish were mainly enriched in biological processes associated with response to stress and stimulus. The KEGG enrichment analysis and pathway-pathway network analysis showed that the MAPK signalling pathway and nine other pathways were the key pathways associated with anti-inflammatory effect. A protein-protein interactions (PPI) network of target genes was constructed, and AKT2 and nine other genes were identified as the hub genes by cytoHubba. Combining the above results, we constructed an active ingredient-hub protein-key pathway network, showing that wogonin-AKT2 was the key regulated module. Molecular docking and SPR-based assays were used to investigate the possible interaction of wogonin with the AKT2 protein. The infected zebrafish and grass carp treated with wogonin displayed a higher survival rate and anti-inflammatory and antioxidant effects than the infected fish without wogonin treatment. Dietary wogonin also enhanced grass carp anti-inflammatory and antioxidant effects. These findings further reveal the molecular biological mechanism by which wogonin from SR exerts anti-inflammatory effects and provide a theoretical basis for the prevention of fish diseases.

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