Systematic Engineering of Saccharomyces cerevisiae Chassis for Efficient Flavonoid-7-O-Disaccharide Biosynthesis
文献类型： 外文期刊
作者： Xiao, Zhiqiang 1 ; Wang, Yongtong 1 ; Liu, Juan 1 ; Zhang, Siqi 1 ; Tan, Xinjia 1 ; Zhao, Yifei 1 ; Mao, Jiwei 4 ; Jiang, Ning 2 ; Zhou, Jingwen 5 ; Shan, Yang 1 ;
作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China
2.Hunan Acad Agr Sci, Agr Prod Proc Inst, Changsha 410125, Peoples R China
3.Hunan Agr Prod Proc Inst, Hunan Key Lab Fruits & Vegetables Storage Proc Qua, Changsha 410125, Peoples R China
4.Chalmers Univ Technol, Dept Life Sci, SE-41296 Gothenburg, Sweden
5.Jiangnan Univ, Sci Ctr Future Foods, Wuxi 214122, Jiangsu, Peoples R China
关键词： flavonoid glycosides; glycosylation; two-enzymesystem; promoter engineering; UDP-glucose
期刊名称：ACS SYNTHETIC BIOLOGY （ 影响因子：4.7； 五年影响因子：4.8 ）
ISSN： 2161-5063
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Flavonoids are an essential class of secondary metabolitesfoundin plants and possess various nutritional, medicinal, and agriculturalproperties. However, the poor water solubility of flavonoid aglyconeslimits their potential applications. To overcome this issue, glycosylationis a promising approach for improving water solubility and bioavailability.In this study, we constructed a flavonoid-7-O-disaccharidebiosynthetic pathway with flavonoid aglycones as substrates in Saccharomyces cerevisiae. Subsequently, through metabolicengineering and promoter strategies, we constructed a UDP-rhamnoseregeneration system and optimized the UDP-glucose (UDPG) syntheticpathway. The optimized strain produced up to 131.3 mg/L eriocitrin.After this, the chassis cells were applied to other flavonoids, withsubstrates such as (2S)-naringenin, (2S)-hesperetin, diosmetin, and (2S)-eriodictyol, whichresulted in the synthesis of 179.9 mg/L naringin, 276.6 mg/L hesperidin,249.0 mg/L neohesperidin, 30.4 mg/L diosmin, and 100.7 mg/L neoeriocitrin.To the best of our knowledge, this is the first report on the biosynthesisof flavonoid-7-O-disaccharide.
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