Systems Metabolic Engineering of Saccharomyces cerevisiae for the High-Level Production of (2S)-Eriodictyol
文献类型： 外文期刊
作者： Zhang, Siqi 1 ; Liu, Juan 1 ; Xiao, Zhiqiang 1 ; Tan, Xinjia 1 ; Wang, Yongtong 1 ; Zhao, Yifei 1 ; Jiang, Ning 1 ; Shan, Yang 1 ;
作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China
2.Hunan Acad Agr Sci, Agr Prod Proc Inst, Changsha 410125, Peoples R China
3.Hunan Agr Prod Proc Inst, Hunan Key Lab Fruits & Vegetables Storage Proc Qua, Changsha 410125, Peoples R China
4.Chalmers Univ Technol, Dept Life Sci, SE-41296 Gothenburg, Sweden
关键词： flavonoid; (2S)-naringenin; (2S)-eriodictyol; metabolic engineering; metabolic balance
期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）
ISSN：
年卷期： 2024 年 10 卷 2 期
页码：
收录情况： SCI
摘要： (2S)-eriodictyol (ERD) is a flavonoid widely found in citrus fruits, vegetables, and important medicinal plants with neuroprotective, cardioprotective, antidiabetic, and anti-obesity effects. However, the microbial synthesis of ERD is limited by complex metabolic pathways and often results in a low production performance. Here, we engineered Saccharomyces cerevisiae by fine-tuning the metabolism of the ERD synthesis pathway. The results showed that the ERD titer was effectively increased, and the intermediate metabolites levels were reduced. First, we successfully reconstructed the de novo synthesis pathway of p-coumaric acid in S. cerevisiae and fine-tuned the metabolic pathway using promoter engineering and terminator engineering for the high-level production of (2S)-naringenin. Subsequently, the synthesis of ERD was achieved by introducing the ThF3 ' H gene from Tricyrtis hirta. Finally, by multiplying the copy number of the ThF3 ' H gene, the production of ERD was further increased, reaching 132.08 mg L-1. Our work emphasizes the importance of regulating the metabolic balance to produce natural products in microbial cell factories.
- 相关文献
作者其他论文 更多>>
-
Bioinspired Janus starch film with dual functionality via citral nanoemulsion-mediated interfacial self-assembly for fresh-cut fruits and vegetables preservation
作者：Xie, Ying;Ding, Ke;Zhang, Shikai;Xu, Saiqing;Li, Huan;Lin, Shuhua;Shan, Yang;Ding, Shenghua;Xie, Ying;Ding, Ke;Xu, Saiqing;Shan, Yang;Ding, Shenghua;Sun, Yuying;Li, Yawen;Wang, Rongrong
关键词：Starch film; Janus structure; Rapid self-assembly; Fresh-cut fruits and vegetables preservation
-
Microbes: Drivers of Chenpi manufacturing, biotransformation, and physiological effects
作者：Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Gao, Zhipeng
关键词：Chenpi; Manufacturing; Biotransformation; Physiological effects; Microbiome; Applications
-
Effects of rolling on eating quality, starch structure, and water distribution in cooked indica rice dough
作者：Xiong, Ying;Zhang, Yu;Yi, Cuiping;Zhu, Hong;Zhang, Yu;Shan, Yang;Fang, Zhongxiang
关键词：rolling process; cooked rice dough; sheeting; starch structure; water status
-
Delaying quality deterioration with multifunctional gelatin-based film by inhibiting microbial growth in fresh-cut navel oranges
作者：Xie, Ying;Xu, Saiqing;Ding, Ke;Xu, Haishan;Qiao, Xinbei;Tang, Qi;Shan, Yang;Ding, Shenghua;Xie, Ying;Xu, Saiqing;Ding, Ke;Xu, Haishan;Qiao, Xinbei;Tang, Qi;Wang, Zijun;Shan, Yang;Ding, Shenghua;Yang, Haiying;Wang, Rongrong;Wang, Zijun;Xu, Yanqun;Ding, Shenghua
关键词：Fresh-cut navel oranges; Multifunctional gelatin-based film; Flavor; Microbial community; Correlation analysis
-
Dynamic changes in the chemical structure and gelling properties of pectin at different stages of citrus maturation and storage
作者：Du, Yuyi;Zhao, Chengying;Wang, Jirong;Zheng, Jinkai;Bao, Yuming;Shan, Yang;Zheng, Jinkai
关键词：Citrus; Pectin; Maturity and storage; Chemical structure; Gelling properties
-
Effect of kaempferol from Acori Tatarinowii Rhizoma (ATR) on grass carp (Ctenopharyngodon idella) infected with Aeromonas hydrophila
作者：Jiang, Ning;Cui, Jun;Fan, Qin;Zhang, Ruoxiao;Zhao, Zihao;Hu, Yibo;Yu, Ziquan;Fan, Qin
关键词：Acori Tatarinowii Rhizoma; Kaempferol; JUN; Anti-inflammatory; Antioxidant; Grass carp
-
Engineering Saccharomyces cerevisiae for De Novo Biosynthesis of 3′-Hydroxygenistein
作者：Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Shan, Yang;Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Liu, Juan;Shan, Yang;Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Liu, Juan;Shan, Yang;Mao, Jiwei
关键词：3 '-hydroxygenistein; genistein; 2-hydroxyisoflavanonesynthase; Saccharomyces cerevisiae; metabolic engineering
