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Association Mapping and Functional Analysis of Rice Cold Tolerance QTLs at the Bud Burst Stage

文献类型： 外文期刊

作者： Wang, Dan 1 ; Liu, Zhuo 1 ; Xiao, Yinghui 1 ; Liu, Xionglun 1 ; Chen, Yue 2 ; Zhang, Zhuo 2 ; Kang, Houxiang 5 ; Wang, Xu 1 ;

作者机构： 1.Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China

2.Hunan Acad Agr Sci, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

3.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China

4.Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA

5.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Rice; Cold tolerance; Bud burst stage; Genome-wide association study

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2021 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Cold tolerance at the bud burst stage (CTB) is a key trait for direct-seeded rice. Although quantitative trait loci (QTL) affecting CTB in rice have been mapped using traditional linkage mapping and genome-wide association study (GWAS) methods, the underlying genes remain unknown. In this study, we evaluated the CTB phenotype of 339 cultivars in the Rice Diversity Panel II (RDP II) collection. GWAS identified four QTLs associated with CTB (qCTBs), distributed on chromosomes 1-3. Among them, qCTB-1-1 overlaps with Osa-miR319b, a known cold tolerance micro RNA gene. The other three qCTBs have not been reported. In addition, we characterised the candidate gene OsRab11C1 for qCTB-1-2 that encodes a Rab protein belonging to the small GTP-binding protein family. Overexpression of OsRab11C1 significantly reduced CTB, while gene knockout elevated CTB as well as cold tolerance at the seedling stage, suggesting that OsRab11C1 negatively regulates rice cold tolerance. Molecular analysis revealed that OsRab11C1 modulates cold tolerance by suppressing the abscisic acid signalling pathway and proline biosynthesis. Using RDP II and GWAS, we identified four qCTBs that are involved in CTB and determined the function of the candidate gene OsRab11C1 in cold tolerance. Our results demonstrate that OsRab11C1 is a negative regulator of cold tolerance and knocking out of the gene by genome-editing may provide enhanced cold tolerance in rice.

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