Identification and characterization of heat-responsive microRNAs at the booting stage in two rice varieties, 9311 and Nagina 22
文献类型： 外文期刊
作者： Luo, Ying 1 ; Wang, Tao 1 ; Yang, Dan 1 ; Luo, Biao 1 ; Wang, Wei-Ping 3 ; Yu, Dong 3 ; He, Fu-Lin 2 ; Wang, Qi-Ming 1 ; Rao 1 ;
作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410125, Peoples R China
2.Hunan Univ Sci & Engn, Coll Chem & Bioengn, Yongzhou 425199, Peoples R China
3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
关键词： rice; heat-responsive; microRNA; target gene; booting stage; high-throughput sequencing
期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）
ISSN： 0831-2796
年卷期： 2021 年 64 卷 11 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are small, non-coding, regulatory RNAs that play important roles in abiotic stress responses in plants, but their regulatory roles in the adaptive response to heat stress at the booting stage in two rice varieties, 9311 and Nagina 22, remain largely unknown. In this study, 464 known miRNAs and 123 potential novel miRNAs were identified. Of these miRNAs, a total of 90 differentially expressed miRNAs were obtained with 9311 libraries as the control group, of which 54 were upregulated and 36 were downregulated. To gain insight into functional significance, 2773 potential target genes of these 90 differentially expressed miRNAs were predicted. GO enrichment analysis showed that the predicted target genes of differentially expressed miRNAs included NACs, LACs, CSD, and Hsp40. KEGG pathway analysis showed that the target genes of these differentially expressed miRNAs were significantly enriched in the plant hormone signal transduction pathway. The expression levels of 10 differentially expressed miRNAs and their target genes obtained by qRT-PCR were largely consistent with the sequencing results. This study lays a foundation for the elucidation of the miRNA-mediated regulatory mechanisms in rice at elevated temperatures.
- 相关文献
作者其他论文 更多>>
-
Improvement of Rice Seed Storability by Regulating Lipid Metabolism Using a CRISPR/Cas9 System
作者：Zhou, Tianshun;Wu, Liubing;Li, Xiaoxiu;Sun, Zhizhong;Yuan, Dingyang;Zhou, Tianshun;Yu, Dong;Tan, Yongjun;Sun, Zhizhong;Sun, Xuewu;Chen, Jin;Yuan, Dingyang;Yu, Dong;Tan, Yongjun;Sun, Zhizhong;Sun, Xuewu;Chen, Jin;Duan, Meijuan;Yuan, Dingyang;Yuan, Dingyang;Duan, Meijuan
关键词：rice; seedstorability; CRISPR/Cas9; lipid metabolism
-
In situ images of Cd2+in rice reveal Cd2+protective mechanism using DNAzyme fluorescent probe
作者：Chen, Jin;Liu, Jianbing;Yu, Dong;Sun, Zhizhong;Sheng, Xiabing;Tan, Yanning;Tan, Yongjun;Sun, Xuewu;Tang, Ning;Wang, Weiping;Tang, Wenbang;Yuan, Dingyang;Duan, Meijuan;Fan, Fan;Hu, Wenjie;Ye, Nenghui;Liu, Zhi;Pan, Jiafeng;Chen, Junhua
关键词：Rice; Cd2+; Cd2+-dependent DNAzyme fluorescence probe; In situ image; Compartmentalized body
-
Establishment of a novel engineering breeding technology for cytoplasmic male sterility
作者：Xu, Na;Yuan, Zheming;Yu, Dong;Zhang, Longhui;Wang, Tiankang;Hu, Zhongxiao;Song, Shufeng;Li, Li;Xu, Na;Yu, Dong;Song, Shufeng;Li, Li;Lei, Quanqiang;Qiu, Ngxin;Song, Shufeng;Li, Li
关键词：Three-line hybrid rice; CMS-WA; Rf gene; CEBT; Reproductive lines
-
Determining rapeseed lodging angles and types for lodging phenotyping using morphological traits derived from UAV images
作者：Wang, Chufeng;Xu, Shijie;You, Yunhao;Zhang, Jian;Liu, Bin;You, Liangzhi;Wang, Chufeng;Xu, Shijie;You, Yunhao;Zhang, Jian;Liu, Bin;Yang, Chenghai;Kuai, Jie;Xie, Jing;Zuo, Qingsong;Yan, Mingli;Du, Hai;Ma, Ni;You, Liangzhi;Wang, Tao;Wu, Hao
关键词：Crop lodging; Total lodging angle; Root lodging angle; Lodging types; Unmanned aerial vehicle
-
Soybean protein isolate inhibiting the retrogradation of fresh rice noodles: Combined experimental analysis and molecular dynamics simulation
作者：Xu, Li;Yi, Cuiping;Wang, Tao;Wang, Ren;Shan, Yang
关键词：Fresh rice noodles; Soybean protein isolate; Fermented indica rice starch; Retrogradation; Molecular dynamics simulation
-
Structural variations of a new fertility restorer gene, Rf20, , underlie the restoration of wild abortive-type cytoplasmic male sterility in rice
作者：Song, Shufeng;Li, Yixing;Qiu, Mudan;Xu, Na;Li, Bin;Chen, Weijun;Wang, Tiankang;Yu, Dong;Pan, Yi;Yuan, Dingyang;Li, Li;Qiu, Mudan;Gong, Mengmeng;Xia, Siqi;Xu, Na;Zhang, Longhui;Li, Lei;Li, Jinglei;Qiu, Yingxin;Dong, Hao;Yuan, Dingyang;Li, Li
关键词：COX11; cytoplasmic male sterility; rf20; WA352; wild abortion
-
Novel CRISPR/Cas9 system assisted by fluorescence marker and pollen killer for high-efficiency isolation of transgene-free edited plants in rice
作者：Yu, Dong;Zhou, Tianshun;Xu, Na;Sun, Xuewu;Song, Shufeng;Liu, Hai;Sun, Zhizhong;Lv, Qiming;Chen, Jin;Tan, Yanning;Sheng, Xiabing;Li, Li;Yuan, Dingyang;Zhou, Tianshun;Li, Li;Yuan, Dingyang
关键词：CRISPR/ Cas9; Transgene-free; Fluorescence Marker; Pollen Killer; Rice
