文献类型： 外文期刊
作者： Xu, Na 1 ; Yu, Dong 2 ; Zhang, Longhui 2 ; Wang, Tiankang 2 ; Hu, Zhongxiao 2 ; Lei, Quanqiang 4 ; Qiu, Ngxin 4 ; Yuan, Zheming 1 ; Song, Shufeng 2 ; Li, Li 2 ;
作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Hunan Engn & Technol Res Ctr Agr Big Data Anal & D, Changsha 410128, Hunan, Peoples R China
2.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China
3.Yuelushan Lab, Changsha 410128, Hunan, Peoples R China
4.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Hunan, Peoples R China
关键词： Three-line hybrid rice; CMS-WA; Rf gene; CEBT; Reproductive lines
期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）
ISSN： 2095-5421
年卷期： 2025 年 13 卷 4 期
页码：
收录情况： SCI
摘要： The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can pro-duce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT con-verts the traditional three-line system into an efficient 'new two-line' hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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