Improvement of Rice Seed Storability by Regulating Lipid Metabolism Using a CRISPR/Cas9 System
文献类型： 外文期刊
作者： Zhou, Tianshun 1 ; Yu, Dong 2 ; Wu, Liubing 1 ; Tan, Yongjun 2 ; Li, Xiaoxiu 1 ; Sun, Zhizhong 1 ; Sun, Xuewu 2 ; Chen, Jin 2 ; Duan, Meijuan 3 ; Yuan, Dingyang 1 ;
作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Hunan, Peoples R China
2.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
3.Yuelushan Lab, Changsha 410128, Peoples R China
4.Minist Agr & Rural Affairs China, Key Lab Biol & Genet Breeding Saline Alkali Tolera, Changsha 410125, Peoples R China
5.Hunan Womens Univ, Changsha 410004, Hunan, Peoples R China
关键词： rice; seedstorability; CRISPR/Cas9; lipid metabolism
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 33 期
页码：
收录情况： SCI
摘要： Long-term storage of rice grain is critical for global food security, yet rice is inherently susceptible to deterioration during storage. Herein, rice seed storability was improved by targeting three key enzyme genes in the lipid metabolism pathway via CRISPR/Cas9 technology, and the mechanism underlying this was analyzed by an untargeted lipidomic approach. Our findings demonstrate that the significantly inferior seed storability in the Yu-Zhen-Xiang (YZX) cultivar compared with the Xi-Li-Gong-Mi (XLGM) cultivar arises from accelerated lipid catabolism and reactive oxygen species (ROS) overproduction. Moreover, a fad2-1/lox3/pld alpha 1 triple mutant in the YZX background was rapidly generated by FMPKC systems, and the mutant exhibited lower fatty acid accumulation and reduced ROS content, along with improved grain quality and nutritional value after accelerated aging. Lipidomic analysis indicated that diminished lipid hydrolysis and peroxidation collectively accounted for enhanced storability of the flp mutant. Collectively, this study establishes a robust strategy for rapidly and significantly improving rice aging tolerance, with potential applicability to other cereal crops for addressing critical challenges of grain storage.
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