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Novel Salinity-Tolerant Third-Generation Hybrid Rice Developed via CRISPR/Cas9-Mediated Gene Editing

文献类型： 外文期刊

作者： Sheng, Xiabing 1 ; Ai, Zhiyong 1 ; Tan, Yanning 1 ; Hu, Yuanyi 1 ; Guo, Xiayu 1 ; Liu, Xiaolin 1 ; Sun, Zhizhong 1 ; Yu, Dong 1 ; Chen, Jin 1 ; Tang, Ning 1 ; Duan, Meijuan 2 ; Yuan, Dingyang 1 ;

作者机构： 1.Natl Ctr Technol Innovat Saline Alkali Tolerant Ri, Hunan Hybrid Rice Res Ctr, Changsha 410125, Peoples R China

2.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China

3.Hunan Acad Agr Sci, Changsha 410125, Peoples R China

4.Sanya Natl Ctr Technol Innovat Saline Alkali Toler, Sanya 572019, Peoples R China

关键词： CRISPR/Cas9; heterosis utilization; OsRR22; rice; salinity tolerance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 9 期

页码：

收录情况： SCI

摘要： Climate change has caused high salinity in many fields, particularly in the mud flats in coastal regions. The resulting salinity has become one of the most significant abiotic stresses affecting the world's rice crop productivity. Developing elite cultivars with novel salinity-tolerance traits is regarded as the most cost-effective and environmentally friendly approach for utilizing saline-alkali land. To develop a highly efficient green strategy and create novel rice germplasms for salt-tolerant rice breeding, this study aimed to improve rice salinity tolerance by combining targeted CRISPR/Cas9-mediated editing of the OsRR22 gene with heterosis utilization. The novel alleles of the genic male-sterility (GMS) and elite restorer line (733S(rr22)-T1447-1 and HZ(rr22)-T1349-3) produced 110 and 1 bp deletions at the third exon of OsRR22 and conferred a high level of salinity tolerance. Homozygous transgene-free progeny were identified via segregation in the T2 generation, with osrr22 showing similar agronomic performance to wild-type (733S and HZ). Furthermore, these two osrr22 lines were used to develop a new promising third-generation hybrid rice line with novel salinity tolerance. Overall, the results demonstrate that combining CRISPR/Cas9 targeted gene editing with the "third-generation hybrid rice system" approach allows for the efficient development of novel hybrid rice varieties that exhibit a high level of salinity tolerance, thereby ensuring improved cultivar stability and enhanced rice productivity.

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