Application of the FLP/LoxP-FRT recombination system to switch the eGFP expression in a model prokaryote
文献类型： 外文期刊
作者： Dan, Junhao 2 ; Deng, Huafeng 1 ; Xia, Yumei 2 ; Zhan, Yijie 2 ; Tang, Ning 1 ; Wang, Yao 2 ; Cao, Mengliang 1 ;
作者机构： 1.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, 736 Yuanda Rd, Changsha 410125, Hunan, Peoples R China
2.Hunan Univ, Longping Branch, Grad Sch, 2 Lushan South Rd, Changsha 410082, Hunan, Peoples R China
关键词： FLP; LoxP-FRT; gene switch; Escherichia coli; eGFP; prokaryotic
期刊名称：OPEN LIFE SCIENCES （ 影响因子：1.311； 五年影响因子：1.293 ）
ISSN： 2391-5412
年卷期： 2022 年 17 卷 1 期
页码：
收录情况： SCI
摘要： In prokaryotes, few studies have applied the flippase (FLP)/P1-flippase recombination target (LoxP-FRT) recombination system to switch gene expression. This study developed a new method for switching gene expression by constructing an FLP/LoxP-FRT site-specific recombination system in Escherichia coli. To this end, we placed the Nos terminator flanked by a pair of LoxP-FRT in front of enhanced green fluorescent protein (eGFP). The Nos terminator was used to block the expression of the eGFP. When a plasmid expressing FLP was available, deletion of the Nos terminator would allow expression of eGFP. The regulatory effect was demonstrated by eGFP expression. The efficiency of the gene switch was calculated as high as 89.67%. The results showed that the FLP/LoxP-FRT recombinase system could be used as a gene switch to regulate gene expression in prokaryotes. This new method for switching gene expression could simplify the gene function analysis in E. coli and other prokaryotes, as well as eukaryotes.
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