文献类型： 外文期刊

作者： Liu, Chang ¹ ; Ma, Tian ³ ; Yuan, Dingyang ⁴ ; Zhou, Yang ⁶ ; Long, Yan ¹ ; Li, Ziwen ¹ ; Dong, Zhenying ¹ ; Duan, Meijuan ⁵ ; Yu, Dong ⁵ ; Jing, Yizhi ¹ ; Bai, Xiaoyue ¹ ; Wang, Yanbo ¹ ; Hou, Quancan ¹ ; Liu, Shuangshuang ¹ ; Zhang, Jin-Song ⁶ ; Chen, Shou-Yi ⁶ ; Li, Dayong ⁷ ; Liu, Xue ⁷ ; Li, Zhikang ⁸ ; Wang, Wensheng ⁸ ; Li, Jinping ² ; Wei, Xun ¹ ; Ma, Biao ³ ; Wan, Xiangyuan ¹ ;

作者机构： 1.Univ Sci & Technol Beijing, Res Ctr Biol & Agr, Shunde Grad Sch, Zhongzhi Int Inst Agr Biosci, Beijing, Peoples R China

2.Beijing Solidwill Sci Tech Co Ltd, Beijing Engn Lab Main Crop Biotech Breeding, Beijing Int Sci & Technol Cooperat Base Biotech B, Beijing, Peoples R China

3.South China Agr Univ, Coll Agr, Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China

4.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Peoples R China

5.Hunan Agr Univ, Coll Agron, Changsha, Peoples R China

6.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing, Peoples R China

7.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing, Peoples R China

8.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China

关键词： grain size and wright; OsEIL1; OsERF115; regulatory module; rice

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）

ISSN： 1467-7644

年卷期： 2022 年 20 卷 8 期

页码：

收录情况： SCI

摘要： Grain size is one of the essential determinants of rice yield. Our previous studies revealed that ethylene plays an important role in grain-size control; however, the precise mechanism remains to be determined. Here, we report that the ethylene response factor OsERF115 functions as a key downstream regulator for ethylene-mediated grain development. OsERF115 encodes an AP2/ERF-type transcriptional factor that is specifically expressed in young spikelets and developing caryopses. Overexpression of OsERF115 significantly increases grain length, width, thickness and weight by promoting longitudinal elongation and transverse division of spikelet hull cells, as well as enhancing grain-filling activity, whereas its knockout mutations lead to the opposite effects, suggesting that OsERF115 positively regulates grain size and weight. OsERF115 transcription is strongly induced by ethylene, and OsEIL1 directly binds to the promoter to activate its expression. OsERF115 acts as a transcriptional repressor to directly or indirectly modulate a set of grain-size genes during spikelet growth and endosperm development. Importantly, haplotype analysis reveals that the SNP variations in the EIN3-binding sites of OsERF115 promoter are significantly associated with the OsERF115 expression levels and grain weight, suggesting that natural variations in the OsERF115 promoter contribute to grain-size diversity. In addition, the OsERF115 orthologues are identified only in grass species, implying a conserved and unique role in the grain development of cereal crops. Our results provide insights into the molecular mechanism of ethylene-mediated grain-size control and a potential strategy based on the OsEIL1-OsERF115-target gene regulatory module for genetic improvement of rice yield.