文献类型： 外文期刊
作者： Liu, Zhuoxin 1 ; Zhang, Chi 1 ; Ma, Jiejia 1 ; Peng, Qianze 2 ; Du, Xiaohua 2 ; Sun, Shu'e 2 ; Cheng, Ju'e 2 ; Peng, Weiye 2 ; Chen, Lijie 1 ; Gu, Zepei 2 ; Zhang, Weixing 2 ; Su, Pin 1 ; Zhang, Deyong 1 ;
作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410082, Peoples R China
2.Hunan Acad Agr Sci, Inst Plant Protect, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
3.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China
4.Natl Ctr Technol Innovat Saline Alkali Tolerant Ri, Sanya 572024, Peoples R China
关键词： extraction microbiota; microbiota diversity; sonication; oscillation and processing times
期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）
ISSN：
年卷期： 2024 年 12 卷 2 期
页码：
收录情况： SCI
摘要： The soil microbiome plays a key role in plant health. Native soil microbiome inoculation, metagenomic profiling, and high-throughput cultivation require efficient microbe extraction. Sonication and oscillation are the most common methods used to extract soil microbiomes. However, the extraction efficiency of these methods has not been investigated in full. In this study, we compared the culturable microbe numbers, community structures, and alpha diversities among the different methods, including sonication, oscillation, and centrifugation, and their processing times. The study results showed that sonication significantly increases the culturable colony number compared with oscillation and centrifugation. Furthermore, the sonication strategy was found to be the main factor influencing extraction efficiency, but increased sonication time can aid in recovery from this impact. Finally, the extraction processing times were found to have a significant negative relationship with alpha-diversity among the extracted microbiota. In conclusion, sonication is the main factor for enriching in situ microbiota, and increased extraction time significantly decreases the alpha-diversity of the extracted microbiota. The results of this study provide insights into the isolation and utilization of different microorganism sources.
- 相关文献
作者其他论文 更多>>
-
Host metabolites explain microbiome variation between different rice genotypes
作者：Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Kang, Houxiang;Peng, Qianze;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Zhang, Chi;Lei, Ziling;Liu, Yong;Zhang, Deyong;Tang, Qianjun;Cernava, Tomislav
关键词：Plant genotype; Plant microbiome; Genetic control; Plant metabolites; Phenylpropanoids
-
Analysis of the Antagonistic Effect of Dibutyl Phthalate on Magnaporthe oryzae through Bioassay and Omics Data
作者：Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue
关键词：dibutyl phthalate; antifungal activity; Magnaportheoryzae; transcriptome; metabolome
-
Transcriptome Analysis Reveals the Role of OsCBM1 in Rice Defense Against Xanthomonas oryzae pv.oryzae
作者：Bie, Shuaijun;Liu, Yong;Zhang, Deyong;Xiao, Youlun;Liu, Yong;Peng, Jing;Gao, Yang;Li, Xiaojuan;Tan, Xinqiu;Huang, Renyan;Zhang, Deyong;Zhang, Li;He, Xiaomin;Xie, Hongjun;Huang, Renyan;Zhang, Deyong
关键词：malectin-like domain-containing protein;
OsCBM1 ;Xanthomonas oryzae pv. oryzae ; reactive oxygen species; phytohormones; rice -
A viral movement protein mediates host volatile biosynthesis to co-attract vectors and non-vectors and enhances viral infection
作者：Li, Shijun;Luo, Xiangwen;Zhang, Zhanhong;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Zhou, Xuguo;Wang, Xin;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Qu, Xiaoxin
关键词：pepper veinal yellows virus; vector; non-vector; volatiles; tetra-interaction
-
Microbiome Migration from Soil to Leaves in Maize and Rice
作者：Ma, Jiejia;Chen, Silu;Liu, Zhuoxin;Zhang, Chi;Lei, Ziling;Su, Pin;Zhang, Deyong;Liu, Yong;Ma, Jiejia;Peng, Qianze;Chen, Silu;Liu, Zhuoxin;Zhang, Weixing;Zhang, Chi;Du, Xiaohua;Sun, Shue;Peng, Weiye;Lei, Ziling;Zhang, Limei;Su, Pin;Zhang, Deyong;Liu, Yong;Ma, Jiejia;Peng, Qianze;Chen, Silu;Liu, Zhuoxin;Zhang, Weixing;Zhang, Chi;Du, Xiaohua;Sun, Shue;Peng, Weiye;Lei, Ziling;Zhang, Limei;Su, Pin;Zhang, Deyong;Liu, Yong
关键词：plant microbiome; soil microbiome; synthetic microbial community; rice; maize; Burkholderiales
-
The effect of developmental stages on microbiome assembly in the phyllosphere and rhizosphere of rice grown in urban area soil
作者：Peng, Qianze;Zhang, Deyong;Peng, Qianze;Sun, Shu'e;Du, Xiaohua;Zhu, Feiying;Peng, Weiye;Liu, Yong;Su, Pin;Zhang, Deyong;Peng, Qianze;Sun, Shu'e;Du, Xiaohua;Zhu, Feiying;Peng, Weiye;Liu, Yong;Su, Pin;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Ma, Jiejia;Chen, Silu;Gao, Liming;Liu, Yong;Su, Pin;Zhang, Deyong;Liu, Xian;Cernava, Tomislav
关键词：Phyllosphere; Rhizosphere; Urbanization; Rice; Microbiome assembly; Plant Microbiome
-
The nematicidal activity of Bacillus thuringiensis Cry1Ia36 expressing in Escherichia coli
作者：Cheng, Feixue;Wang, Dongwei;Long, Minzhi;Sun, Shu-e;Zhu, Chunhui;Cheng, Ju-e;Tan, Xinqiu;Zhang, Deyong;Liu, Yong;Wang, Jian;Wang, Xin
关键词：Plant-parasitic nematodes; Meloidogyne incognita; Bacillus thuringiensis; Cry1Ia36 protein; Nematicidal activity; Interaction proteins
