Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9
文献类型： 外文期刊
作者： Zhou, Yanbiao 1 ; Xu, Shichong 1 ; Jiang, Nan 1 ; Zhao, Xinhui 1 ; Bai, Zhenan 1 ; Liu, Jinling 4 ; Yao, Wei 4 ; Tang, Qianying 1 ; Xiao, Gui 5 ; Lv, Chao 1 ; Wang, Kai 1 ; Hu, Xiaochun 1 ; Tan, Junjie 6 ; Yang, Yuanzhu 1 ;
作者机构： 1.Yuan Longping High Tech Agr Co Ltd, Minist Agr & Rural Affairs, Hunan Engn Lab Dis & Pest Resistant Rice Breeding, Key Lab Southern Rice Innovat & Improvement, Changsha 410001, Hunan, Peoples R China
2.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China
4.Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China
5.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China
6.Nanjing Agr Univ, Innovat Ctr Genome Editing & Engn, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
关键词： rice; TGMS; CRISPR; Cas9; rice blast; bacterial blight
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）
ISSN： 1467-7644
年卷期： 2022 年 20 卷 5 期
页码：
收录情况： SCI
摘要： Rice blast and bacterial blight represent two of major diseases having devastating impact on the yield of rice in most rice-growing countries. Developments of resistant cultivars are the most economic and effective strategy to control these diseases. Here, we used CRISPR/Cas9-mediated gene editing to rapidly install mutations in three known broad-spectrum blast-resistant genes, Bsr-d1, Pi21 and ERF922, in an indica thermosensitive genic male sterile (TGMS) rice line Longke638S (LK638S). We obtained transgene-free homozygous single or triple mutants in T-1 generations. While all single and triple mutants showed increased resistance to rice blast compared with wild type, the erf922 mutants displayed the strongest blast resistance similar with triple mutants. Surprisingly, we found that Pi21 or ERF922 single mutants conferred enhanced resistance to most of tested bacterial blight. Both resistances in mutants were attribute to the up-regulation of SA- and JA-pathway associated genes. Moreover, phenotypic analysis of these single mutants in paddy fields revealed that there were no trade-offs between resistances and main agricultural traits. Together, our study provides a rapid and effective way to generate rice varieties with resistance to both rice blast and bacterial blight.
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