The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont
文献类型： 外文期刊
作者： Liu, Ying 1 ; Qin, Ziwei 1 ; Chen, Ning 1 ; Bu, Zhigang 1 ; Yang, Yuanzhu 2 ; Hu, Xiaochun 2 ; Zheng, Heping 3 ; Zhu, Zhuoyi 1 ; Xu, Ting 1 ; Gao, Yan 1 ; Niu, Shuqi 1 ; Xing, Junjie 4 ; Lin, Jianzhong 1 ; Liu, Xuanming 1 ; Zhu, Yonghua 1 ;
作者机构： 1.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China
2.Yahua Seeds Sci Acad Hunan, State Key Lab Hybrid Rice, Changsha 410000, Hunan, Peoples R China
3.Hunan Univ, Coll Biol, Bioinformat Ctr, Changsha 410082, Hunan, Peoples R China
4.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China
关键词： endophytic Streptomyces; rice; thiamine biosynthesis; THIC; blast resistance
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.895； 五年影响因子：6.048 ）
ISSN： 0021-8561
年卷期： 2022 年 70 卷 23 期
页码：
收录情况： SCI
摘要： Endophytes can benefit the growth and stress resistance of host plants by secreting bioactive components. Thiamine is an essential vitamin involved in many metabolic pathways and can only be synthesized by microbes and plants. In this study, we found that thiamine could inhibit the development of the phytopathogen Magnaporthe oryzae and decrease the rice blast index under field conditions. In the thiamine biosynthesis pathway, the key enzyme ShTHIC of an endophyte Streptomyces hygroscopicus OsiSh-2 and OsTHIC of rice (Oryza sativa) were highly homologous. Gene overexpression or knockout approaches revealed that both THIC contributed to thiamine synthesis and resistance to M. oryzae. Furthermore, S. hygroscopicus OsiSh-2 colonization led to a decrease in the thiamine synthesis level of rice but still maintained thiamine homeostasis in rice. However, inoculation with the ShTHIC knockout strain Delta THIC reduced the thiamine content in rice, although the thiamine synthesis level of rice was increased. After infection with M. oryzae, blast resistance was dramatically improved in OsiSh-2-inoculated rice but decreased in Delta THIC-inoculated rice compared with non-inoculated rice. This result demonstrated that ShTHIC could regulate thiamine biosynthesis and consequently assist blast resistance in the OsiSh-2-rice symbiont. Our results revealed a novel blast-resistance mechanism mediated by a key thiamine biosynthetic enzyme from an endophyte OsiSh-2.
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