Optimizing Production of Pectinase from Orange Peel by Penicillium oxalicum PJ02 Using Response Surface Methodology
文献类型： 外文期刊
作者： Li, Pei-Jun 1 ; Xia, Jin-Lan 1 ; Shan, Yang 2 ; Nie, Zhen-Yuan 1 ; Su, Dong-Lin 2 ; Gao, Qi-Rui 1 ; Zhang, Chuang 4 ; Ma, 1 ;
作者机构： 1.Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China
2.Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China
3.Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China
4.Cent S Univ, Longping Branch Grad Sch, Changsha 410125, Hunan, Peoples R China
关键词： Pectinase;Cellulolytic enzymes;Orange peel;Response surface methodology
期刊名称：WASTE AND BIOMASS VALORIZATION （ 影响因子：3.703； 五年影响因子：3.624 ）
ISSN： 1877-2641
年卷期： 2015 年 6 卷 1 期
页码：
收录情况： SCI
摘要： A novel strain Penicillium oxalicum PJ02 was isolated for the production of exo-pectinase, endo-pectinase, carboxymethylcellulase (CMCase) and xylanase. Response surface methodology (RSM) was employed to optimize orange peel submerged fermentation for pectinase production. Plackett-Burman factorial design was used to select significant parameters. Temperature and NH4Cl concentration had significant influence on exo-pectinase production. The central composite design was used to generate a total of 22 fermentation experiments. The statistical results indicated that the optimum temperature for exo-pectinase and endo-pectinase production was 36.5 degrees C, and optimal NH4Cl concentration was 1.12 g/L. Under this condition, the yield of exo-pectinase was 36.88 U/mL, endo-pectinase was 0.62 U/mL, which are in close agreement with the values predicted by the model. The CMCase and xylanase yield in the same medium were 0.38 and 0.57 U/mL, respectively. RSM was also used to analyse the effect of independent variables on CMCase and xylanase production.
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