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RELATIONSHIP OF ASCORBIC ACID METABOLISM WITH THE CYTOPLASMIC MALE STERILITY IN PEPPER (CAPSICUM ANNUUM L.)

文献类型: 外文期刊

作者: Wen, Jinfen 1 ; Deng, Minghua 1 ; Huo, Jinlong 2 ; Zhu, Haishan 2 ; Dai, Xiongze 3 ; Zhang, Zhuqing 3 ; Lv, Junheng 2 ; Z 1 ;

作者机构: 1.Kunming Univ Sci & Technol, Kunming 650224, Peoples R China

2.Yunnan Agr Univ, Kunming 650201, Peoples R China

3.Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China

关键词: Capsicum annuum L.;Mitochondrion;Cytoplasmic male sterility;Metabolism of ascorbic acid;Gene expression

期刊名称:PAKISTAN JOURNAL OF BOTANY ( 影响因子:0.972; 五年影响因子:0.988 )

ISSN: 0556-3321

年卷期: 2015 年 47 卷 3 期

页码:

收录情况: SCI

摘要: To elucidate the metabolic mechanism of Ascorbic Acid (ASA) in the CMS-pepper anthers, the metabolism changes in the reactive oxygen species (ROS) contents, antioxidants contents and ROS scavenging enzymatic activities were investigated in the anther mitochondria of CMS-9704A and maintainer-9704B. At the abortion stage, anthers of CMS-9704A had higher contents of ROS than those of the maintainer. Simultaneously, there were lower contents of ASA and glutathione (GSH) in stage 2 and 3, and the lower activities of ascorbate peroxidase (APX) and glutathione perodixase (GPX) in stage 3 in scavenging ROS in the anthers of the CMS line than maintainer. The expression level of APX and GPX in stage 3 in anthers of CMS-9704A was obviously inhibited when ROS produced with a great deal during anther stage; however the gene expression kept normal in the maintainer. Excessive accumulation of ROS, significant reduction activities and gene expression level of ROS-scavenging enzyme were coinstantaneous with CMS.

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