Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis
文献类型： 外文期刊
作者： Yang, Chunxiao 1 ; Li, Hui 2 ; Pan, Huipeng 3 ; Ma, Yabin 2 ; Zhang, Deyong 1 ; Liu, Yong 1 ; Zhang, Zhanhong 4 ; Zheng, 1 ;
作者机构： 1.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China
2.Qingdao Agr Univ, Coll Agron & Plant Protect, Qingdao, Peoples R China
3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
4.Hunan Acad Agr Sci, Hunan Vegetable Inst, Changsha, Hunan, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 8 期
页码：
收录情况： SCI
摘要： Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes. To facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. The western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), the main vector of tomato spotted wilt virus (TSWV), is a destructive invasive species. In this study, the expression profiles of 11 candidate reference genes from nonviruliferous and viruliferous F. occidentalis were investigated. Five distinct algorithms, geNorm, NormFinder, BestKeeper, the Delta C-t method, and RefFinder, were used to determine the performance of these genes. geNorm, NormFinder, BestKeeper, and RefFinder identified heat shock protein 70 (HSP70), heat shock protein 60 (HSP60), elongation factor 1 alpha, and ribosomal protein l32 (RPL32) as the most stable reference genes, and the Delta C-t method identified HSP60, HSP70, RPL32, and heat shock protein 90 as the most stable reference genes. Additionally, two reference genes were sufficient for reliable normalization in nonviruliferous and viruliferous F. occidentalis. This work provides a foundation for investigating the molecular mechanisms of TSWV and F. occidentalis interactions.
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