Selection of reference genes for RT-qPCR analysis in a predatory biological control agent, Coleomegilla maculata (Coleoptera: Coccinellidae)
文献类型： 外文期刊
作者： Yang, Chunxiao 2 ; Pan, Huipeng 2 ; Noland, Jeffrey Edward 2 ; Zhang, Deyong 1 ; Zhang, Zhanhong 3 ; Liu, Yong 1 ; Zho 1 ;
作者机构： 1.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China
2.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
3.Hunan Acad Agr Sci, Hunan Vegetable Inst, Changsha, Hunan, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2015 年 5 卷
页码：
收录情况： SCI
摘要： Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying gene expression across various biological processes, of which requires a set of suited reference genes to normalize the expression data. Coleomegilla maculata (Coleoptera: Coccinellidae), is one of the most extensively used biological control agents in the field to manage arthropod pest species. In this study, expression profiles of 16 housekeeping genes selected from C. maculata were cloned and investigated. The performance of these candidates as endogenous controls under specific experimental conditions was evaluated by dedicated algorithms, including geNorm, Normfinder, BestKeeper, and Delta Ct method. In addition, RefFinder, a comprehensive platform integrating all the above-mentioned algorithms, ranked the overall stability of these candidate genes. As a result, various sets of suitable reference genes were recommended specifically for experiments involving different tissues, developmental stages, sex, and C. maculate larvae treated with dietary double stranded RNA. This study represents the critical first step to establish a standardized RT-qPCR protocol for the functional genomics research in a ladybeetle C. maculate. Furthermore, it lays the foundation for conducting ecological risk assessment of RNAi-based gene silencing biotechnologies on non-target organisms; in this case, a key predatory biological control agent.
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