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Pepper vein yellow virus P0 protein triggers NbHERC3, NbBax, and NbCRR mediated hypersensitive response

文献类型： 外文期刊

作者： Ouyang, Xian 1 ; Wang, Lishuang 2 ; Luo, Xiangwen 3 ; Li, Chun 2 ; An, Xingyu 2 ; Yao, Ling 2 ; Huang, Wei 2 ; Zhang, Zhanhong 3 ; Zhang, Songbai 1 ; Liu, Yong 1 ; Wu, Shiping 2 ;

作者机构： 1.Hunan Agr Univ, Plant Protect Coll, Changsha, Peoples R China

2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

3.Hunan Acad Agr Sci, Inst Plant Protect, Key Lab Pest Management Hort Crop Hunan Prov, Changsha, Peoples R China

4.Hunan Agr Univ, Plant Protect Coll, Changsha 410128, Peoples R China

5.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550000, Peoples R China

关键词： hypersensitive response; jasmonic acid; P0 protein; pepper vein yellow virus; salicylic acid

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：3.1； 五年影响因子：3.0 ）

ISSN： 0233-111X

年卷期： 2024 年 64 卷 6 期

页码：

收录情况： SCI

摘要： P0 proteins encoded by the pepper vein yellow virus (PeVYV) are pathogenic factors that cause hypersensitive response (HR). However, the host gene expression related to PeVYV P0-induced HR has not been thoroughly studied. Transcriptomic technology was used to investigate the host pathways mediated by the PeVYV P0 protein to explore the molecular mechanisms underlying its function. We found 12,638 differentially expressed genes (DEGs); 6784 and 5854 genes were significantly upregulated and downregulated, respectively. Transcriptomic and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) analyses revealed that salicylic acid (SA) and jasmonic acid (JA) synthesis-related gene expression was upregulated, and ethylene synthesis-related gene expression was downregulated. Ultrahigh performance liquid chromatography-tandem mass spectrometry was used to quantify SA and JA concentrations in Nicotiana benthamiana, and the P0 protein induced SA and JA biosynthesis. We then hypothesized that the pathogenic activity of the P0 protein might be owing to proteins related to host hormones in the SA and JA pathways, modulating host resistance at different times. Viral gene silencing suppression technology was used in N. benthamiana to characterize candidate proteins, and downregulating NbHERC3 (Homologous to E6-AP carboxy-terminus domain and regulator of choromosome condensation-1 dmain protein 3) accelerated cell necrosis in the host. The downregulation of NbCRR reduced cell death, while that of NbBax induced necrosis and curled heart leaves. Our findings indicate that NbHERC3, NbBax, and NbCRR are involved in P0 protein-driven cell necrosis.

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