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Quantitative analysis of ethylene precursor ACC in plant samples by liquid chromatography-tandem mass spectrometry

文献类型： 外文期刊

作者： Tong, Jianhua 1 ; Zhao, Wenkui 2 ; Wang, Keming 3 ; Zhu, Feiying 4 ; Deng, Danyi 1 ; Xiao, Langtao 1 ;

作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China

2.Hunan Agr Univ, Coll Chem & Mat Sci, Changsha 410128, Peoples R China

3.Hunan Agr Univ, Assets & Lab Management Dept, Changsha 410128, Peoples R China

4.Hunan Acad Agr Sci, Changsha 410125, Peoples R China

关键词： Ethylene; 1-Aminocyclopropane-1-carboxylic acid; Phytohormone; Tandem mass spectrometry; Liquid-liquid micro-extraction; Precise control of the mobile phase

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundEthylene (C2H4) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also considered as a plant growth regulator. Accurate quantification of ACC is critically important in investigating its function. However, it remains challenging to accurately quantify ACC in plant tissues because it is a small, electroneutral molecule with very low concentrations.MethodsAn easy, cost-saving, and highly efficient quantitative method for ACC in plant tissues was set up by liquid-liquid micro-extraction (LLME) purification with green solvent ethyl-acetate, and the precise control of the mobile phase entering into the mass spectrometer combined with the ultra-high performance liquid chromatography electrospray ionization-triple quadrupole mass spectrometer (UHPLC-ESI-MS/MS).ResultsThe contents of ACC in 10 mg of different fresh fruits were detected. The established method had limit of detection (LOD) (2.5 pg), matrix effect (ME) (92.6%), good precision (3.54%), recovery rate (95.82%), and a good linear relationship within the range of 0.5 to 1500 ng.mL-1 (R2 = 0.9998).ConclusionWe have developed an easy, sensitive, and cost-saving quantitative method for ACC in plant tissues without derivatization which is useful for the precise quantification of ACC in plant tissues.

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