Safety Evaluation and Whole Genome Sequencing of Aspergillus japonIicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing
文献类型： 外文期刊
作者： Qian, Yujiao 1 ; Gao, Zhipeng 3 ; Wang, Jieyi 1 ; Wang, Chen 1 ; Li, Gaoyang 1 ; Fu, Fuhua 1 ; Guo, Jiajing 1 ; Shan, Yan 1 ;
作者机构： 1.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China
2.Hunan Acad Agr Sci, Int Joint Lab Fruits & Vegetables Proc, Qual & Safety,Hunan Agr Prod Proc Inst, Hunan Key Lab Fruits & Vegetables Storage Proc Qu, Changsha 410125, Peoples R China
3.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China
关键词： Aspergillus japonicas PJ01; segments degradation; gizzard juice process; safety evaluation; genome sequencing
期刊名称：FOODS （ 影响因子：4.35； 五年影响因子：4.957 ）
ISSN：
年卷期： 2021 年 10 卷 8 期
页码：
收录情况： SCI
摘要： Aspergillus japonicas PJ01 (A. japonicas PJ01) is a strain isolated from the rotten branches. In previ-ous studies, it was shown that it can produce complex enzymes to degrade polysaccharide com-ponents. In this study, we evaluated the safety of its crude enzyme solution. Acute oral toxicity, subchronic toxicity, micronucleus and sperm malformation tests all validated the high biologi-cal safety for the crude enzymes. Secondly, we carried out the citrus segment degradation ex-periment of crude enzyme solution. Compared with the control group, the crude enzyme solu-tion of A. japonicas PJ01 can completely degrade the segments in 50 min, which provides the basis for enzymatic peeling during juice processing. The whole genome sequencing showed that the genome of A. japonicus PJ01 has a GC content of 51.37% with a size of 36204647 bp, and encoded 10070 genes. GO, COG, KEGG and CAZy databases were used in gene annotation analyses. Pathway enrichment showed many genes related to carbohydrate metabolism, rich in genes re-lated to pectinase, xylanase and carboxylcellulase. Therefore, the complex enzyme produced by A. japonicus PJ01 can be used in gizzard juice processing to achieve efficient enzymatic decapsu-lation.
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