您好，欢迎访问湖南省农业科学院 机构知识库！

Transcriptome profiling and functional analysis suggest that the constitutive overexpression of four cytochrome P450s confers resistance to abamectin in Tetranychus urticae from China

文献类型： 外文期刊

作者： Xu, Dandan 1 ; Zhang, Yan 3 ; Zhang, Youjun 3 ; Wu, Qingjun 3 ; Guo, Zhaojiang 3 ; Xie, Wen 3 ; Zhou, Xiaomao 1 ; Wang, S 1 ;

作者机构： 1.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, 12 Zhongguancun Nandajie, Beijing 100081, Peoples R China

关键词： Tetranychus urticae; abamectin‐ resistance; transcriptome; P450; overexpression

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND The two-spotted spider mite Tetranychus urticae is a polyphagous and cosmopolitan pest that has developed high resistance to abamectin, making it difficult to control. Although 'target resistance' related to glutamate-gated chloride channel mutations was found in T. urticae field populations in China, other resistance mechanisms appear to be involved. Here, we conducted genome-wide transcriptome profiling using RNA-sequencing of two abamectin-resistant populations (NB-ZJ and SY-BJ) and one susceptible strain (Lab-SS) to identify differentially expressed genes that might contribute to the resistance of T. urticae to abamectin in China. RESULTS Our experiments showed that abamectin resistance was synergized by piperonyl butoxide (PBO) and triphenyl phosphate (TPP), with synergistic ratios (SR) of 2.95-fold and 2.21-fold for PBO and 3.55-fold and 2.84-fold for TPP in NB-ZJ and SY-BJ populations, respectively. Transcriptome data and quantitative real-time PCR (qRT-PCR) revealed that seven detoxification enzyme genes were overexpressed in the two resistant populations. Furthermore, functional analysis by RNA interference (RNAi) indicated that the mortality caused by abamectin was significantly increased by the separate silencing of the P450 genes CYP389C10, CYP392D8, CYP392A11, and CYP392A12. CONCLUSION qRT-PCR expression and RNAi data suggest that the overexpression of P450 genes CYP389C10, CYP392D8, CYP392A11, and CYP392A12 may be involved in the abamectin-resistance of field populations of T. urticae in China. This knowledge could facilitate the elucidation of resistance mechanisms and the development of resistance management of T. urticae field populations.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台