RNAi-mediated knockdown of fruitless in Plutella xylostella (Lepidoptera: Plutellidae) disrupts female sex pheromone biosynthesis and male courtship behavior
文献类型： 外文期刊
作者： Huang, Zizhou 1 ; Liu, Zheming 2 ; Ma, Haihao 1 ; Zhou, Yong 2 ; Zhao, Piao 2 ; Zhu, Hang 2 ; Liu, Jia 1 ; Man, Yilong 2 ; Zhou, Xiaomao 1 ;
作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China
2.Hunan Acad Agr Sci, Hunan Inst Plant Protect, Changsha 410125, Peoples R China
3.Hunan Prov Key Lab Pesticide Biol & Precise Use Te, Changsha, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Pesticide Assessment, Changsha 410125, Peoples R China
5.Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China
关键词： fruitless; alternative splicing; courtship behavior; sex pheromone
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.4； 五年影响因子：2.4 ）
ISSN： 0022-0493
年卷期： 2025 年 118 卷 3 期
页码：
收录情况： SCI
摘要： Strong fecundity is an important reason why the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), has become one of the most serious pests of cruciferous vegetables worldwide. Disrupting with courtship and mating behaviors has emerged as an important strategy for insect management. The fruitless (fru) gene encodes a transcription factor that contains a BTB (Broad-Complex, Tramtrack and Bric a brac) and a zinc finger pair related to the C2H2 class. It plays a crucial role in regulating insect courtship behavior. In this study, the fru gene of P. xylostella (Pxfru) was cloned, revealing 7 alternative splicing forms (Pxfru-1 to Pxfru-7). Pxfru-1 to Pxfru-3 were non-sex-specific transcripts, while the remaining forms were male-specific. Subcellular localization experiments demonstrated that the transcripts encoding proteins containing BTB and zinc finger domains (Pxfru-1 to Pxfru-3) localized to the cell nucleus, whereas Pxfru-4 and Pxfru-5, which contain only one BTB domain, were localized in the nucleus and cytoplasm, respectively. Knockdown the expression of fru in male moths delayed occurrence of mating and reduced their preference to female sex pheromones. Meanwhile, suppression of fru expression in female P. xylostella decreased their attractiveness to males. The results of GC-MS and Y-tube olfaction experiments indicated that this change may be attributed to alterations in the proportion of sex pheromones. This study represents the first report of the fru gene influencing pheromone ratios in female insects, and provides a new perspective for understanding the function of fru in the courtship behavior of non-model insects.
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