beta-Cyclodextrin Inclusion Complex Containing Litsea cubeba Essential Oil: Preparation, Optimization, Physicochemical, and Antifungal Characterization
文献类型： 外文期刊
作者： Wang, Yinhong 1 ; Yin, Chunxiao 1 ; Cheng, Xiaomei 1 ; Li, Gaoyang 2 ; Shan, Yang 2 ; Zhu, Xiangrong 1 ;
作者机构： 1.Hunan Univ, Longping Branch Grad Sch, Changsha 410125, Peoples R China
2.Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Hunan Prov Key Lab Fruits & Vegetables Storage Pr, Changsha 410125, Peoples R China
关键词： Litsea cubeba essential oil; inclusion complex; response surface methodology; antifungal activity; postharvest disease
期刊名称：COATINGS （ 影响因子：2.881； 五年影响因子：3.038 ）
ISSN：
年卷期： 2020 年 10 卷 9 期
页码：
收录情况： SCI
摘要： Litsea cubeba essential oil (LCEO), as naturally plant-derived products, possess good antimicrobial activities against many pathogens, but their high volatility and poor water solubility limit greatly the application in food industry. In this research, inclusion complex based on beta-cyclodextrin (beta-CD) and LCEO, was prepared by saturated aqueous solution method. An optimum condition using the response surface methodology (RSM) based on Box-Behnken design (BBD) was obtained with the inclusion time of 2 h and beta-CD/LCEO ratio of 4.2 at 44 degrees C. Under the condition, the greatest yield of 71.71% with entrapment efficiency of 33.60% and loading capacity of 9.07% was achieved. In addition, the structure and characteristic of LCEO/beta-CD inclusion complex (LCEO/beta CD-IC) were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR), which indicated that LCEO/beta CD-IC was successfully formed. The particle size of LCEO/beta CD-IC was determined to be 17.852 mu m. Thermal properties of LCEO/beta CD-IC evaluated by thermogravimetric-differential scanning calorimetry (TG-DTA) illustrated better thermal stability of the aimed product compared with the physical mixture. Furthermore, the tests of antifungal activity showed that LCEO/beta CD-IC was able to control the growth of Penicillium italicum, Penicillium digitatum, and Geotrichum citri-aurantii isolated from postharvest citrus. Our present study confirmed that LCEO/beta CD-IC might be further applied as an alternative to chemical fungicides for protecting citrus fruit from postharvest disease.
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