Analysis of the Antifungal Properties ofBacillus velezensisB-4 Through a Bioassay and Complete-Genome Sequencing
文献类型: 外文期刊
作者: Zhu, Zheyuan 1 ; Peng, Qiong 2 ; Man, Yilong 2 ; Li, Zuren 2 ; Zhou, Xiaomao 2 ; Bai, Lianyang 3 ; Peng, Di 2 ;
作者机构: 1.Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China
2.Hunan Agr Biotechnol Res Inst, Changsha, Peoples R China
3.Hunan Acad Agr Sci CAAS, Changsha, Peoples R China
关键词: antibacterial activity; complete genome; comparative genome; Bacillus velezensisB-4; biology control
期刊名称:FRONTIERS IN GENETICS ( 影响因子:4.599; 五年影响因子:4.888 )
ISSN:
年卷期: 2020 年 11 卷
页码:
收录情况: SCI
摘要: The strain B-4, isolated from a field in Changsha (China), presents strong antifungal activities, as identified by the Kirby-Bauer test, especially for pathogens that harm crops. Here, we obtained the complete genome sequence of the strain B-4 by Pacific Biosciences single-molecule real-time sequencing, making it well analyzed for understanding mechanisms and creating biological agents. Its 3,919-kb circular chromosome genome has 3,725 protein-coding genes [coding sequences (CDSs)] and 46.7% guanine-cytosine content. A comparative genome analysis of B-4 with other published strains (includingBacillus velezensis,Bacillus amyloliquefaciens, andBacillus subtilis) revealed that the strain B-4 is aB. velezensisstrain. These different strains have 2,889 CDSs in common, whereas 179 CDSs were found to be unique in the strain B-4, which is a far greater number than that in other strains. Regarding the antifungal activities of B-4, we were specifically concerned with the genes involved in the biosynthesis of secondary metabolites. In total, more than 19.56% of the genome was annotated to 12 gene clusters relating to synthesis of antimicrobial metabolites, which contained various enzyme-encoding operons for non-ribosomal peptide synthetases, polyketide synthases, and lantipeptide synthesis proteins. They were all considered to be related to the production of bacteriostatic substances or stimulation of induced systemic resistance by bacterial metabolites. These situations also present an advantage over those of other strains for biocontrol potential. We provide evidence that the biological control effect of the strain B-4, as demonstrated in antibacterial activity experiments and predicted from the complete genome sequence analysis, provides the basis for research promoting agricultural research on sustainable development, especially the contribution of biotechnology to agriculture.
- 相关文献
作者其他论文 更多>>
-
Self-Assembled Sphere Covalent Organic Framework with Enhanced Herbicidal Activity by Loading Cyhalofop-butyl
作者:Deng, Xile;Bai, Lianyang;Zhao, Pengyue;Xie, Yong
关键词:covalent organic framework; nanopesticide; high loads; enhanced bioactivity; sustainable agriculture
-
Root Endophyte Shift and Key Genera Discovery in Rice under Barnyardgrass Stress
作者:Li, Shuyan;Yan, Qiling;Wang, Jieyu;Jiang, Huidan;Li, Zuren;Peng, Qiong;Li, Shuyan;Jiang, Huidan;Li, Zuren;Peng, Qiong
关键词:rice; Echinochloa crus-galli; biotic stress; endophytic bacterium; endophytic fungus; phylogenetic investigation of communities by reconstruction of unobserved states; FUNGuild test
-
Generating herbicide resistant and dwarf rice germplasms through precise sequence insertion or replacement
作者:Liu, Xiaoxia;Li, Ming;Wei, Chuang;Dong, Yinghui;Xue, Li;Zhu, Jian-Kang;Liu, Xiaoxia;Li, Ming;Wei, Chuang;Dong, Yinghui;Xue, Li;Zhu, Jian-Kang;Liu, Xiaoxia;Wang, Yingying;Wang, Hongzhi;He, Yuxin;Wang, Mugui;Wang, Yingying;Wang, Hongzhi;He, Yuxin;Wang, Mugui;Wang, Yingying;Wang, Hongzhi;He, Yuxin;Song, Yijiao;Li, Zuren;Zhang, Jinshan;Zhu, Jian-Kang;Zhu, Jian-Kang
关键词:prime editing; targeted insertion; targeted replacement; herbicide resistant
-
Multi-omics analyses reveal the crosstalk between the circadian clock and the response to herbicide application in Oryza sativa
作者:Chen, Ke;Yang, Haona;Peng, Yajun;Wu, Lamei;Zhao, Zhenghong;Bai, Lianyang;Wang, Lifeng;Chen, Ke;Zhao, Zhenghong;Bai, Lianyang;Wang, Lifeng;Chen, Ke;Yang, Haona;Peng, Yajun;Wu, Lamei;Zhao, Zhenghong;Bai, Lianyang;Wang, Lifeng;Su, Xiao;Lin, Tao
关键词:Oryza sativa L; circadian clock; herbicides; RNA-seq; metabolome
-
A MOF-based pH-responsive dual controlled-release system for herbicide pretilachlor and safener AD-67 delivery that enhances the herbicidal efficacy and reduces side effects
作者:Deng, Xile;Peng, Yajun;Bai, Lianyang;Guo, Kunyan;Yang, Ni;Qian, Kun
关键词:
-
Phytochemical profiling and allelopathic effect of garlic essential oil on barnyard grass (Echinochloa crusgalli L.)
作者:Bai, Haodong;Ni, Xianzhi;Han, Jincai;Luo, Dingfeng;Jin, Chenzhong;Li, Zuren;Bai, Haodong;Ni, Xianzhi;Hu, Yihong;Jin, Chenzhong;Li, Zuren
关键词:
-
Room-temperature ultrasonic-assisted self-assembled synthesis of silkworm cocoon-like COFs@GCNTs composite for sensitive detection of diuron in food samples
作者:Li, Dongdong;Bai, Lianyang;Li, Dongdong;Bai, Lianyang;Li, Dongdong;Zhao, Hongyuan;Wang, Guoquan;Liu, Runqiang
关键词:Diuron; Electrochemical sensor; Synergistic effect; Covalent organic frameworks; Graphitized multi -walled carbon nanotubes