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Analysis of the Antifungal Properties ofBacillus velezensisB-4 Through a Bioassay and Complete-Genome Sequencing

文献类型： 外文期刊

作者： Zhu, Zheyuan 1 ; Peng, Qiong 2 ; Man, Yilong 2 ; Li, Zuren 2 ; Zhou, Xiaomao 2 ; Bai, Lianyang 3 ; Peng, Di 2 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China

2.Hunan Agr Biotechnol Res Inst, Changsha, Peoples R China

3.Hunan Acad Agr Sci CAAS, Changsha, Peoples R China

关键词： antibacterial activity; complete genome; comparative genome; Bacillus velezensisB-4; biology control

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN：

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The strain B-4, isolated from a field in Changsha (China), presents strong antifungal activities, as identified by the Kirby-Bauer test, especially for pathogens that harm crops. Here, we obtained the complete genome sequence of the strain B-4 by Pacific Biosciences single-molecule real-time sequencing, making it well analyzed for understanding mechanisms and creating biological agents. Its 3,919-kb circular chromosome genome has 3,725 protein-coding genes [coding sequences (CDSs)] and 46.7% guanine-cytosine content. A comparative genome analysis of B-4 with other published strains (includingBacillus velezensis,Bacillus amyloliquefaciens, andBacillus subtilis) revealed that the strain B-4 is aB. velezensisstrain. These different strains have 2,889 CDSs in common, whereas 179 CDSs were found to be unique in the strain B-4, which is a far greater number than that in other strains. Regarding the antifungal activities of B-4, we were specifically concerned with the genes involved in the biosynthesis of secondary metabolites. In total, more than 19.56% of the genome was annotated to 12 gene clusters relating to synthesis of antimicrobial metabolites, which contained various enzyme-encoding operons for non-ribosomal peptide synthetases, polyketide synthases, and lantipeptide synthesis proteins. They were all considered to be related to the production of bacteriostatic substances or stimulation of induced systemic resistance by bacterial metabolites. These situations also present an advantage over those of other strains for biocontrol potential. We provide evidence that the biological control effect of the strain B-4, as demonstrated in antibacterial activity experiments and predicted from the complete genome sequence analysis, provides the basis for research promoting agricultural research on sustainable development, especially the contribution of biotechnology to agriculture.

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