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Expression and alternative splicing analysis of a large-conductance calcium-activated potassium channel gene inPlutella xylostella

文献类型： 外文期刊

作者： Xu, Jie 1 ; Ma, Hai-Hao 2 ; Liu, Zhe-Ming 2 ; Zheng, Wei 2 ; Lai, Xiao-Yi 2 ; Zhu, Hang 2 ; Liu, Jia 2 ; Zhou, Yong 2 ; Zhou 1 ;

作者机构： 1.Hunan Agr Univ, Grad Sch, Coll Plant Protect, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha, Peoples R China

关键词： alternative splicing; BKCa; gene expression pattern; IBTX; Plutella xylostella

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

ISSN： 0739-4462

年卷期： 2020 年 105 卷 1 期

页码：

收录情况： SCI

摘要： The large-conductance calcium-activated potassium channel (BKCa) plays an important role in the regulation of insect neural circuits and locomotion, and thus is a potential target of insecticides. In this study, iberiotoxin, an inhibitor of BKCa, was found to prolong the anesthetic time of ethyl acetate onPlutella xylostellalarvae. Therefore, the coding sequence of slowpoke gene coding the alpha subunit of BK(Ca)was cloned to investigate the function of this channel inP. xylostella, and the gene expression profile in the developmental stages and tissues was also characterized. The total length ofpxsloDNA was more than 19.9 kb, which harbored four alternative splicing sites (ASP-A, ASP-C, ASP-E, and ASP-G), and the coding sequence ofpxslowith the highest frequency of splicing (GenBank ID: MN938456) was 3,405 base pair. The characterized PxSlo protein contained conserved domains previously identified in other insects. Quantitative reverse transcription-polymerase chain reaction analysis showed thatpxslowas expressed in all the developmental stages ofP. xylostella, with the highest level in adults. In the larval stage,pxslowas mainly expressed in the head and epidermis, while a limited protein was expressed in the midgut. In the adult stage,pxslowas highly expressed in the head, followed by in the ovarian tubule, and was not expressed in the testis or wings. These results suggest that BK(Ca)plays an important physiological role inP. xylostellaand provides useful information for the functional study and screening of BK(Ca)inhibitors.

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