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Transcription factor OsSGL is a regulator of starch synthesis and grain quality in rice

文献类型： 外文期刊

作者： Liu, Zhenming 1 ; Jiang, Shun 3 ; Jiang, Lingli 1 ; Li, Wanjing 3 ; Tang, Yuqin 3 ; He, Wei 1 ; Wang, Manling 5 ; Xing, Junjie 4 ; Cui, Yanchun 5 ; Lin, Qinlu 3 ; Yu, Feng 1 ; Wang, Long 1 ;

作者机构： 1.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

2.Hunan Univ, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China

3.Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Changsha 410004, Peoples R China

4.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

5.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China

关键词： Grain quality; OsSGL; OsSUS1; rice; transcriptional activity

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：7.298； 五年影响因子：8.291 ）

ISSN： 0022-0957

年卷期： 2022 年 73 卷 11 期

页码：

收录情况： SCI

摘要： Starch biosynthesis during rice endosperm development is important for grain quality, as it influences grain size and physico-chemical properties, which together determine rice eating quality. Cereal starch biosynthetic pathways have been comprehensively investigated; however, their regulation, especially by transcriptional repressors remains largely unknown. Here, we identified a DUF1645 domain-containing protein, STRESS_tolerance and GRAIN_LENGTH (OsSGL), that participates in regulating rice starch biosynthesis. Overexpression of OsSGL reduced total starch and amylose content in the endosperm compared with the wild type. Chromatin immunoprecipitation sequencing and RNA-seq analyses indicated that OsSGL targets the transcriptional activity of several starch and sucrose metabolism genes. In addition, ChIP-qPCR, yeast one-hybrid, EMSA and dual-luciferase assays demonstrated that OsSGL directly inhibits the expression of SUCROSE SYNTHASE 1 (OsSUS1) in the endosperm. Furthermore, OsSUS1 interacts with OsSGL to release its transcriptional repression ability. Unexpectedly, our results also show that knock down and mutation of OsSGL disrupts the starch biosynthetic pathway, causing lower starch and amylose content. Therefore, our findings demonstrate that accurate control of OsSGL homeostasis is essential for starch synthesis and grain quality. In addition, we revealed the molecular mechanism of OsSGL in regulating starch biosynthesis-related genes, which are required for grain quality. A DUF1645 domain-containing protein, OsSGL, participates in regulating rice starch synthesis and directly inhibits the expression of SUCROSE SYNTHASE 1(OsSUS1) in the endosperm.

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