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Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants

文献类型： 外文期刊

作者： Song, Ge 1 ; Wu, Jia-yu 2 ; Xie, Yan 1 ; Liu, Yong 3 ; Qian, Ya-juan 1 ; Zhou, Xue-ping 1 ; Wu, Jian-xiang 1 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China

3.Chinese Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Potato virus S;Tissue print-enzyme-linked immunosorbent assay (ELISA);Dot-ELISA;Double-antibody sandwich (DAS)-ELISA

期刊名称：JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B （ 影响因子：3.066； 五年影响因子：3.057 ）

ISSN： 1673-1581

年卷期： 2017 年 18 卷 12 期

页码：

收录情况： SCI

摘要： Potato virus S (PVS) often causes significant losses in potato production in potato-growing countries. In this study, the ordinary strain of PVS (PVSO) was purified from PVS-infected potato plants and used as the immunogen to produce hybridomas secreting monoclonal antibodies (MAbs). Five highly specific and sensitive murine MAbs (1A3, 16C10, 18A9, 20B12, and 22H4) against PVS were prepared using conventional hybridoma technology. Using these MAbs, tissue print-enzyme-linked immunosorbent assay (ELISA), dot-ELISA, and double-antibody sandwich (DAS)- ELISA were developed for sensitive and specific detection of PVS infection in potato plants. The results of sensitivity assays revealed that PVS could be reliably detected in PVS-infected leaf crude extracts diluted at 1:10 240 and 1:163 840 (w/v, g/ml) in phosphate buffer saline (PBS) by dot-ELISA and DAS-ELISA, respectively. Twenty-two samples collected from potato fields in Yunnan Province, China were tested for PVS infection using the serological assays we had developed, and 14 of them were found to be positive. This indicates that PVS is now prevalent in potato fields in Yunnan Province.

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