您好，欢迎访问湖南省农业科学院 机构知识库！

Characterization of a Novel Polerovirus Infecting Maize in China

文献类型： 外文期刊

作者： Chen, Sha 1 ; Jiang, Guangzhuang 1 ; Wu, Jianxiang 1 ; Liu, Yong 3 ; Qian, Yajuan 1 ; Zhou, Xueping 1 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Pest Management Hort Crop Hunan Prov, Changsha 410125, Hunan, Peoples R China

关键词： Maize yellow mosaic virus;deep sequencing;infectious clone;suppressor;molecular variation;sRNA

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2016 年 8 卷 5 期

页码：

收录情况： SCI

摘要： A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide sequence identity (73%) to Maize Yellow Dwarf Virus-RMV. Sequence comparisons and phylogenetic analyses suggested that MaYMV represents a new member of the genus Polerovirus in the family Luteoviridae. Furthermore, the P0 protein encoded by MaYMV was demonstrated to inhibit both local and systemic RNA silencing by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene, which further supported the identification of a new polerovirus. The biologically-active cDNA clone of MaYMV was generated by inserting the full-length cDNA of MaYMV into the binary vector pCB301. RT-PCR and Northern blot analyses showed that this clone was systemically infectious upon agro-inoculation into N. benthamiana. Subsequently, 13 different isolates of MaYMV from field-grown maize plants in different geographical locations of Yunnan and Guizhou provinces of China were sequenced. Analyses of their molecular variation indicate that the 3 half of P3-P5 read-through protein coding region was the most variable, whereas the coat protein- (CP-) and movement protein- (MP-)coding regions were the most conserved.

  • 相关文献

[1]Complete genome sequence of a Chinese isolate of pepper vein yellows virus and evolutionary analysis based on the CP, MP and RdRp coding regions. Liu, Xiangning,Li, Xun,Dai, Liangyin,Tang, Qianjun,Liu, Maoyan,Liu, Xiangning,Dai, Liangyin,Tang, Qianjun,Tang, Qianjun,Li, Xun,Zhang, Deyong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台