湖南省农业科学院机构知识库

A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus

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文献类型：外文期刊

作者： Jiang, Mengting ¹ ; Luo, Xiangwen ¹ ; Zhang, Deyong ¹ ; Zhou, Xuguo ⁴ ; Ouyang, Xian ³ ; Zhang, Zhanhong ³ ; Li, Fan;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Pest Management Hort Crop Hunan Prov, Changsha 410125, Hunan, Peoples R China

3.Hunan Acad Agr Sci, Hunan Vegetable Inst, Changsha 410125, Hunan, Peoples R China

4.Univ Kentucky, Dep

关键词： detection;pepper;Pepper vein yellows virus;PeVYV;RT-LAMP;RT-PCR

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （影响因子：2.442；五年影响因子：1.993 ）

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年卷期：

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收录情况： SCI

摘要： Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62 degrees C for 45min and the whole reaction took less than 1h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.

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