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Characterization of Molecular Identity and Pathogenicity of Rice Blast Fungus in Hunan Province of China

文献类型： 外文期刊

作者： Xing, Junjie 1 ; Jia, Yulin 1 ; Peng, Zhirong 2 ; Shi, Yinfeng 2 ; He, Qiang 2 ; Shu, Fu 2 ; Zhang, Wuhan 2 ; Zhang, Zhen 3 ;

作者机构： 1.ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA

2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Rice, Hunan, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China

4.Southern Reg Collaborat Innovat Ctr, Changsha 410128, Hunan, Peoples R China

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

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收录情况： SCI

摘要： The blast (Magnaporthe oryzae) resistance (R) gene is the most economical and environmental method to control rice blast disease. Characterization of molecular identity and pathogenicity of M. oryzae benefits the deployment of effective blast R genes.In order to identify blast R genes that would be effective in Hunan Province, 182 M. oryzae strains were analyzed with a Chinese differential system (CDS), repetitive element-based polymerase chain reaction (rep-PCR), and the presence and absence of avirulence (AVR) genes by PCR amplification with gene-specific primers. Identified blast R genes were validated with 24 monogenic lines (ML) carrying 24 major R genes. In total, 28 races(isolates) of M. oryzae was identified with CDS, and classified into 20 distinct groups with rep-PCR. Interestingly, AVR-Pia, AVR-Pik, AVR-Pizt, AVR-Pib, and AVR-PI9 were detected in more than 86.8% of the isolates; AVR-Pital was in 51.3% and AVR-Pii wasin only 2.5%. In contrast, pathogenicity assays on 24 ML demonstrated that Pi9, Piz5, Pikh, and Pikm were more effective, with resistant frequencies of 91.6, 91, 87.9, and 87.3%, respectively; Pia, Piks, Pit, Pill, and Pib were less than 15%. These findings revealed the complexity of a genetic basis of rice blast resistance, and shed light on useful blast R genes in Hunan Province.

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