Integrated transcriptome and BSA-seq analysis identifies a novel QTL for Meloidogyne graminicola resistance in rice HuaHang31
文献类型： 外文期刊
作者： Yang, Zhuhong 1 ; Xiao, Qingyan 1 ; Wang, Yao 1 ; Zhang, Yingjun 1 ; Ye, Shan 1 ; Sun, Pingyong 2 ; Zhang, Wuhan 2 ; Deng, Huafeng 2 ; Liu, Shiming 3 ; Ding, Zhong 1 ;
作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China
2.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
3.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）
ISSN： 0040-5752
年卷期： 2025 年 138 卷 9 期
页码：
收录情况： SCI
摘要： Key messageA novel QTL on chromosome 11 with 7 candidate genes regulating nematode resistance was identified by RNA-seq-based transcriptome and BSA-seq combination analyses, revealing the possible interactions between nematodes and plants.AbstractThe resistant rice germplasms against Meloidogyne graminicola, which is a plant-parasitic nematode posing a significant threat to rice production, are very limited. Here, we identified HuaHang31 (HH31), an indica rice variety, as a novel source of high resistance against to M. graminicola through large-scale screening of 297 rice accessions using PF-127 assays. Histopathological and histochemical analyses revealed that HH31 significantly reduced nematode penetration, delayed juvenile development, and triggered reactive oxygen species accumulation and hypersensitive response in root tip cells post-infection. RNA-seq-based transcriptome profiling of HH31 and susceptible IR64 roots at 3-, 7-, and 12-days post-inoculation (dpi) highlighted the up-regulation of phenylpropanoid, flavonoid, lignin biosynthesis, and phytohormone signaling pathways (jasmonic acid, salicylic acid, ethylene) in HH31, suggesting their pivotal roles in nematode resistance. Additionally, bulk segregant analysis of two F-2 populations derived from HH31 crosses with susceptible varieties mapped a novel quantitative trait locus (QTL) on chromosome 11 (4.7-8.2 Mb). Within this region, seven nonsynonymous nucleotide-binding site-leucine-rich repeat genes exhibited root-specific expression and responsiveness to nematode infection, implicating their roles in resistance. Importantly, this QTL represents a distinct genomic region compared to previously reported resistance loci, highlighting its novelty. This study provides a new understanding of the genetic basis of nematode resistance and a valuable source for the development of nematode-resistant rice varieties, offering a sustainable strategy to mitigate yield losses caused by this pervasive pathogen.
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