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The genome sequence of Brassica campestris chrysovirus 1, a novel putative plant-infecting tripartite chrysovirus

文献类型: 外文期刊

作者: Zhang, Jun 3 ; Zhao, Zhonghao 1 ; Hu, Rong 1 ; Guo, Lingfang; Zheng, Limin 2 ; Du, Zhenguo; Wu, Zujian; Fang, Sh 1 ;

作者机构: 1.Yangtze Univ, Engn Res Ctr Ecol & Agr Use Wetland, Minist Educ, Jingzhou 434025, Hubei, Peoples R China

2.Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China

3.Yangtze Univ, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Hunan, Peoples R China

4.Fujian Agr &

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

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收录情况: SCI

摘要: A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1'' (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respectively, each containing a single open reading frame (ORF 1-3). The putative proteins encoded by ORF 1-3 show homologies to RdRp, CP and chryso-P3 of approved or tentative chrysoviruses. In addition, the three dsRNAs of BrcCV1 contain highly conserved 5' and 3' untranslated regions (UTRs) in a way similar to known chrysoviruses. In a phylogenetic tree based on the conserved amino acid sequences of the RdRps of chrysoviruses, totiviruses and partitiviruses, the putative BrcCV1 formed a separate clade with Raphanus sativus chrysovirus 1 (RasCV1), a putative trisegmented, plant-infecting chrysovirus, in the family Chrysoviridae.

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