文献类型: 外文期刊
作者: Bu, Fengjiao 1 ; Chen, Huiming; Shi, Qiuxiang 1 ; Zhou, Qian 1 ; Gao, Dongli 1 ; Zhang, Zhonghua 1 ; Huang, Sanwen 1 ;
作者机构: 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Sino Dutch Joint Lab Hort Genom, Minist Agr,Inst Vegetables & Flowers, Beijing 100081, Peoples R China
期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )
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收录情况: SCI
摘要: Cucumber (Cucumis sativus L.) is a model species for sex determination studies, and its yield is associated with the degree of femaleness. Subgynoecy represents a sex form with a high degree of femaleness for which the genetic basis remains elusive. In this study, genetic analysis in the F-2 and BC1 populations developed from a cross between subgynoecious S-2-98 and monoecious M95 suggested a quantitative nature of subgynoecy. Application of simple sequence repeat markers between subgynoecious and monoecious bulks constructed from BC1 plants identified three QTLs: sg3.1, sg6.1, and sg6.2. The major QTL sg3.1 contributed to 54.6 % of the phenotypic variation, and its presence was confirmed by genome-wide comparison of SNP profiles between parental lines and a subgynoecious bulk constructed from BC6 plants. Using PCR-based markers developed from the SNP profile, sg3.1 was further delimited to a genomic region of 799 kb. The genetic basis of subgynoecy revealed here shall shed light on the development of elite cultivars with high yield potential.
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