Targeted creation of new mutants with compact plant architecture using CRISPR/Cas9 genome editing by an optimized genetic transformation procedure in cucurbit plants
文献类型： 外文期刊
作者： Xin, Tongxu 1 ; Tian, Haojie 1 ; Ma, Yalin 1 ; Wang, Shenhao 3 ; Yang, Li 4 ; Li, Xutong 1 ; Zhang, Mengzhuo 2 ; Chen, Chen 5 ; Wang, Huaisong 1 ; Li, Haizhen 6 ; Xu, Jieting 7 ; Huang, Sanwen 2 ; Yang, Xueyong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Sino Dutch Joint Lab Hort Genom, Inst Vegetables & Flowers,Minist Agr, Beijing 100081, Peoples R China
2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518124, Peoples R China
3.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
4.Huazhong Agr Univ, Coll Hort & Forestry Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China
5.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China
6.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Inst, Beijing 100097, Peoples R China
7.Wimi Biotechnol Jiangsu Co Ltd, Changzhou 213000, Jiangsu, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.291； 五年影响因子：7.487 ）
ISSN： 2662-6810
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： Fruits and vegetables in the Cucurbitaceae family, such as cucumber, melon, watermelon, and squash, contribute greatly to the human diet. The widespread use of genome editing technologies has greatly accelerated gene functional characterization and crop improvement. However, most economically important cucurbit plants, including melon and squash, remain recalcitrant to standard Agrobacterium tumefaciens-mediated transformation, limiting the effective use of genome editing technology. In this study, we used an "optimal infiltration intensity" strategy to establish an efficient genetic transformation system for melon and squash. We harnessed the power of this method to target homologs of the ERECTA family of receptor kinase genes and created alleles that resulted in a compact plant architecture with shorter internodes in melon, squash, and cucumber. The optimized transformation method presented here enables stable CRISPR/Cas9-mediated mutagenesis and provides a solid foundation for functional gene manipulation in cucurbit crops.
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