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Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

文献类型： 外文期刊

作者： Li, Pei-jun 1 ; Xia, Jin-lan 1 ; Shan, Yang 2 ; Nie, Zhen-yuan 1 ;

作者机构： 1.Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China

关键词： Multi-enzyme complexes;Solid-state fermentation;Submerged fermentation;Ultrasound;Zymography

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

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收录情况： SCI

摘要： Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

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[1]Pectic oligosaccharides hydrolyzed from orange peel by fungal multi enzyme complexes and their prebiotic and antibacterial potentials. Xia, Jin-lan,Nie, Zhen-yuan,Shan, Yang.

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