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Batch and multi-step fed-batch enzymatic saccharification of Formiline-pretreated sugarcane bagasse at high solid loadings for high sugar and ethanol titers

文献类型: 外文期刊

作者: Zhao, Xuebing 1 ; Dong, Lei 1 ; Chen, Liang 2 ; Liu, Dehua 1 ;

作者机构: 1.Tsinghua Univ, Inst Appl Chem, Dept Chem Engn, Beijing 100084, Peoples R China

2.Hunan Inst Nucl Agr Sci & Space Breeding, Changsha 410125, Hunan, Peoples R China

关键词: Enzymatic saccharification;Fed-batch process;Formiline pretreatment;High solid loading;Lignocellulose

期刊名称:BIORESOURCE TECHNOLOGY ( 影响因子:9.642; 五年影响因子:9.237 )

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收录情况: SCI

摘要: Formiline pretreatment pertains to a biomass fractionation process. In the present work, Formiline-pretreated sugarcane bagasse was hydrolyzed with cellulases by batch and multi-step fed-batch processes at 20% solid loading. For wet pulp, after 144. h incubation with cellulase loading of 10. FPU/g dry solid, fed-batch process obtained ~150. g/L glucose and ~80% glucan conversion, while batch process obtained ~130. g/L glucose with corresponding ~70% glucan conversion. Solid loading could be further increased to 30% for the acetone-dried pulp. By fed-batch hydrolysis of the dried pulp in pH. 4.8 buffer solution, glucose concentration could be 247.3. ±. 1.6. g/L with corresponding 86.1. ±. 0.6% glucan conversion. The enzymatic hydrolyzates could be well converted to ethanol by a subsequent fermentation using Saccharomices cerevisiae with ethanol titer of 60-70. g/L. Batch and fed-batch SSF indicated that Formiline-pretreated substrate showed excellent fermentability. The final ethanol concentration was 80. g/L with corresponding 82.7% of theoretical yield.

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